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CD53 Antibody (161-2 (53/2)) [Allophycocyanin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12076APC

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-12076APC

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 161-2 (53/2)

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for CD53 Antibody (161-2 (53/2)) [Allophycocyanin]

Immunogen

This antibody was raised by immunizng mice with stimulated human leukocytes.

Specificity

This antibody recognizes human CD53. It does not react with the baboon and horse antigen. Cd53 is a member of tetraspanin family and is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Applications for CD53 Antibody (161-2 (53/2)) [Allophycocyanin]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD53

The antibody reacts with CD53 antigen, a glycoprotein of 32-40 kDa belonging to the tetraspans family (TM4SF). CD53 antigen is exclusivelly expressed on leucocytes (not present on platelets, red blood cells and non-hematopoietic cells). CD53 is important for signal transduction. CD53 cross-linking promotes activation of human B cells and rat macrophages.

Alternate Names

CD53, MOX44, TSPAN25

Gene Symbol

CD53

Additional CD53 Products

Product Documents for CD53 Antibody (161-2 (53/2)) [Allophycocyanin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD53 Antibody (161-2 (53/2)) [Allophycocyanin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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