CD53 Antibody (161-2 (53/2)) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12077
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: CD53 Antibody (161-2 (53/2)) - Chimeric - Azide and BSA Free [NBP3-12077]](https://resources.bio-techne.com/images/products/CD53-Antibody-161-2-53-2-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12077-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CD53 Antibody (161-2 (53/2)) - Chimeric - Azide and BSA Free [NBP3-12077]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 161-2 (53/2)
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was raised by immunizng mice with stimulated human leukocytes.
Specificity
This antibody recognizes human CD53. It does not react with the baboon and horse antigen. Cd53 is a member of tetraspanin family and is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for CD53 Antibody (161-2 (53/2)) - Chimeric - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: CD53 Antibody (161-2 (53/2)) - Chimeric - Azide and BSA Free [NBP3-12077]
Immunocytochemistry/Immunofluorescence: CD53 Antibody (161-2 (53/2)) - Chimeric [NBP3-12077] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 161-2 (53/2) (NBP3-12077) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12077, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications for CD53 Antibody (161-2 (53/2)) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: CD53
Alternate Names
CD53, MOX44, TSPAN25
Gene Symbol
CD53
Additional CD53 Products
Product Documents for CD53 Antibody (161-2 (53/2)) - Chimeric - Azide and BSA Free
Product Specific Notices for CD53 Antibody (161-2 (53/2)) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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