CD53 Antibody (HD77) [DyLight 550]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12058R

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-12058R
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

DyLight 550 (Excitation = 562 nm, Emission = 576 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HD77

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD53 Antibodies »

Product Summary for CD53 Antibody (HD77) [DyLight 550]

Immunogen

This antibody was raised by immunising mice with human leukemia cells.

Specificity

This antibody is specific for the extracytoplasmic part of human CD53, a pan-leukocyte antigen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for CD53 Antibody (HD77) [DyLight 550]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD53

The antibody reacts with CD53 antigen, a glycoprotein of 32-40 kDa belonging to the tetraspans family (TM4SF). CD53 antigen is exclusivelly expressed on leucocytes (not present on platelets, red blood cells and non-hematopoietic cells). CD53 is important for signal transduction. CD53 cross-linking promotes activation of human B cells and rat macrophages.

Alternate Names

CD53, MOX44, TSPAN25

Gene Symbol

CD53

Additional CD53 Products

Product Documents for CD53 Antibody (HD77) [DyLight 550]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD53 Antibody (HD77) [DyLight 550]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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