CD53 Antibody (MEM-53) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-393
Clone MEM-53 was used by HLDA to establish CD designation.
![Flow Cytometry: CD53 Antibody (MEM-53) - BSA Free [NB500-393]](https://resources.bio-techne.com/images/products/CD53-Antibody-MEM-53-Flow-Cytometry-NB500-393-img0002.jpg "Flow Cytometry: CD53 Antibody (MEM-53) - BSA Free [NB500-393]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # MEM-53
Format
BSA Free
Concentration
1.0 mg/ml
Product Summary for CD53 Antibody (MEM-53) - BSA Free
Immunogen
Leukocytes of patient suffering from a LGL-type leukemia.
Specificity
The antibody MEM-53 reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F). HLDA IV; WS Code NL 59 HLDA V; WS Code B CD53.5 HLDA V; WS Code BP BP287 HLDA V; WS Code T T-096 HLDA V; WS Code X XB004
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CD53 Antibody (MEM-53) - BSA Free
Flow Cytometry: CD53 Antibody (MEM-53) - BSA Free [NB500-393]
Flow Cytometry: CD53 Antibody (MEM-53) [NB500-393] - Staining of CD53 in human peripheral blood cells with anti-CD53 (MEM-53) PE.![Flow Cytometry: CD53 Antibody (MEM-53) - BSA Free [NB500-393]](https://resources.bio-techne.com/images/products/CD53-Antibody-MEM-53-Flow-Cytometry-NB500-393-img0001.jpg "Flow Cytometry: CD53 Antibody (MEM-53) - BSA Free [NB500-393]")
Flow Cytometry: CD53 Antibody (MEM-53) - BSA Free [NB500-393]
Flow Cytometry: CD53 Antibody (MEM-53) [NB500-393] - Staining of CD53 in human peripheral blood cells with anti-CD53 (MEM-53) Pacific BlueTM.![CD53 Antibody (MEM-53) - BSA Free Flow Cytometry: CD53 Antibody (MEM-53) - BSA Free [NB500-393]](https://resources.bio-techne.com/images/products/nb500-393_mouse-monoclonal-cd53-antibody-mem-53-13120241649379.jpg "Flow Cytometry: CD53 Antibody (MEM-53) - BSA Free [NB500-393]")
Flow Cytometry: CD53 Antibody (MEM-53) - BSA Free [NB500-393]
Separation of human monocytes (red-filled) from human CD53 negative blood debris (black-dashed) in Analysis (surface staining) of human peripheral blood stained using anti-human CD53 (MEM-53) purified antibody (concentration in sample 3 ug/ml, GAM APC).Applications for CD53 Antibody (MEM-53) - BSA Free
Application
Recommended Usage
Flow Cytometry
1-5 ug/ml
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:200
Immunoprecipitation
1:50
Western Blot
1:1000
Application Notes
This antibody is suitable for discrimination of lymphomas from other tumors. Functional Application: The antibody MEM-53 induces activation of monocytes and B lymphocytes. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CD53
Additional CD53 Products
Product Documents for CD53 Antibody (MEM-53) - BSA Free
Product Specific Notices for CD53 Antibody (MEM-53) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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