CD55/DAF Antibody (F4-29D9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47963

Clone F4-29D9 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP2-47963-0.1mg
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # F4-29D9

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all CD55/DAF Antibodies »

Product Specifications

Immunogen

Human umbilical vein endothelial cells (HUVEC)

Localization

Cell Surface

Specificity

Recognizes a single chain glycoprotein of 70kDa, identified as CD55. This monoclonal antibody was clustered in Kobe at the Sixth International Workshop on Human Leukocyte Differentiation Antigens as F429D-9 (N-L120). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. Anti-CD55 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44872).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD55/DAF Antibody (F4-29D9) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD55/DAF Antibody (F4-29D9) - Azide and BSA Free [NBP2-47963]

Immunocytochemistry/ Immunofluorescence: CD55/DAF Antibody (F4-29D9) - Azide and BSA Free [NBP2-47963]

Immunocytochemistry/Immunofluorescence: CD55/DAF Antibody (F4-29D9) - Azide and BSA Free [NBP2-47963] - Staining of paraformaldehyde-fixed HeLa cells with CD55 Mouse Monoclonal Antibody (F4-29D9) followed by goat anti-Mouse IgG-CF488 (Green). Counterstain is RedDot.
Flow Cytometry: CD55/DAF Antibody (F4-29D9) - Azide and BSA Free [NBP2-47963]

Flow Cytometry: CD55/DAF Antibody (F4-29D9) - Azide and BSA Free [NBP2-47963]

Flow Cytometry: CD55/DAF Antibody (F4-29D9) - Azide and BSA Free [NBP2-47963] - Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells with CD55/DAF Antibody (F4-29D9) followed by goat anti-Mouse IgG-CF488 (Blue) Isotype Control (Red)

Applications for CD55/DAF Antibody (F4-29D9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunofluorescence

0.5 - 1.0 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD55/DAF

CD55 encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Alternate Names

CD55, CR, DAF, TC

Gene Symbol

CD55

Additional CD55/DAF Products

Product Documents for CD55/DAF Antibody (F4-29D9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD55/DAF Antibody (F4-29D9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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