CD57 Antibody (NK-1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44352
![Immunohistochemistry-Paraffin: CD57 Antibody (NK-1) [NBP2-44352]](https://resources.bio-techne.com/images/products/beta-1-3-Glucuronyltransferase-1-B3GAT1-Antibody-NK-1-Immunohistochemistry-Paraffin-NBP2-44352-img0001.jpg "Immunohistochemistry-Paraffin: CD57 Antibody (NK-1) [NBP2-44352]")
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat (Negative)
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Kappa Clone # NK-1
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Membrane antigen from HSB-2 cells
Reactivity Notes
Does not react with Rat.
Localization
Cell surface
Specificity
Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.
Marker
Natural Killer Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgM Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47789)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD57 Antibody (NK-1)
Immunohistochemistry-Paraffin: CD57 Antibody (NK-1) [NBP2-44352]
Immunohistochemistry-Paraffin: CD57 Antibody (NK-1) [NBP2-44352] - Human spleen stained with CD57 Monoclonal Antibody (NK-1).Applications for CD57 Antibody (NK-1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1 ug/ml
Immunohistochemistry-Paraffin
2-4 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein L or PEG purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD57
CD57 expression has become a common pathological marker. CD57+ CD8+ T cell expression is observed in various cancers and is often linked to reduced survival (3). On the other hand, CD57+ NK cell expression is associated with less severe disease and better prognosis in cancer patients (3). CD57 is also associated with immune dysfunction and autoimmune disease (3,4). For example, T cell immunosenescence is characterized by an upregulation of CD57 along with KLRG-1 and a loss of CD27 and CD28 expression (4). Expanded autoreactive T cells are also related to diseases including multiple sclerosis (MS) and rheumatoid arthritis (RA) (3). Conversely, reduced numbers of CD57+ NK cells and NK cell cytotoxicity is observed in various autoimmune diseases such as apoptotic dermatitis and sjogren's syndrome (3).
References
1. Focosi D, Bestagno M, Burrone O, Petrini M. CD57+ T lymphocytes and functional immune deficiency. J Leukoc Biol. 2010;87(1):107-116. https://doi.org/10.1189/jlb.0809566
2. Morise J, Takematsu H, Oka S. The role of human natural killer-1 (HNK-1) carbohydrate in neuronal plasticity and disease. Biochim Biophys Acta Gen Subj. 2017;1861(10):2455-2461. https://doi.org/10.1016/j.bbagen.2017.06.025
3. Nielsen CM, White MJ, Goodier MR, Riley EM. Functional Significance of CD57 Expression on Human NK Cells and Relevance to Disease. Front Immunol. 2013;4:422. Published 2013 Dec 9. https://doi.org/10.3389/fimmu.2013.00422
4. Lian J, Yue Y, Yu W, Zhang Y. Immunosenescence: a key player in cancer development. J Hematol Oncol. 2020;13(1):151. Published 2020 Nov 10. https://doi.org/10.1186/s13045-020-00986-z6
Alternate Names
HNK1, HNK-1, Human Natural Killer-1, L2, LEU7, LEU-7
Gene Symbol
B3GAT1
UniProt
Additional CD57 Products
Product Documents for CD57 Antibody (NK-1)
Product Specific Notices for CD57 Antibody (NK-1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...