CD57 Antibody (SPM129)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44353
![Immunohistochemistry-Paraffin: CD57 Antibody (SPM129) [NBP2-44353]](https://resources.bio-techne.com/images/products/CD57-Antibody-SPM129-Immunohistochemistry-Paraffin-NBP2-44353-img0002.jpg "Immunohistochemistry-Paraffin: CD57 Antibody (SPM129) [NBP2-44353]")
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Rat (Negative)
Applications
Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Kappa Clone # SPM129
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Membrane antigen from HSB-2 cells
Reactivity Notes
Does not react with Rat.
Localization
Cell surface
Specificity
Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.
Marker
Natural Killer Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgM Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47790)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD57 Antibody (SPM129)
Immunohistochemistry-Paraffin: CD57 Antibody (SPM129) [NBP2-44353]
Immunohistochemistry-Paraffin: CD57 Antibody (SPM129) [NBP2-44353] - Analysis of CD57 antibody (SPM129) on human spleen. Image from verified customer review.![Immunohistochemistry-Paraffin: CD57 Antibody (SPM129) [NBP2-44353]](https://resources.bio-techne.com/images/products/beta-1-3-Glucuronyltransferase-1-B3GAT1-Antibody-SPM129-Immunohistochemistry-Paraffin-NBP2-44353-img0001.jpg "Immunohistochemistry-Paraffin: CD57 Antibody (SPM129) [NBP2-44353]")
Immunohistochemistry-Paraffin: CD57 Antibody (SPM129) [NBP2-44353]
Immunohistochemistry-Paraffin: CD57 Antibody (SPM129) [NBP2-44353] - Human Spleen stained with CD57 Monoclonal Antibody (SPM129).Applications for CD57 Antibody (SPM129)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 1 ug/ml
Immunohistochemistry-Paraffin
2-4 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Reviewed Applications
Read 1 review rated 5 using NBP2-44353 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein L or PEG purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD57
CD57 expression has become a common pathological marker. CD57+ CD8+ T cell expression is observed in various cancers and is often linked to reduced survival (3). On the other hand, CD57+ NK cell expression is associated with less severe disease and better prognosis in cancer patients (3). CD57 is also associated with immune dysfunction and autoimmune disease (3,4). For example, T cell immunosenescence is characterized by an upregulation of CD57 along with KLRG-1 and a loss of CD27 and CD28 expression (4). Expanded autoreactive T cells are also related to diseases including multiple sclerosis (MS) and rheumatoid arthritis (RA) (3). Conversely, reduced numbers of CD57+ NK cells and NK cell cytotoxicity is observed in various autoimmune diseases such as apoptotic dermatitis and sjogren's syndrome (3).
References
1. Focosi D, Bestagno M, Burrone O, Petrini M. CD57+ T lymphocytes and functional immune deficiency. J Leukoc Biol. 2010;87(1):107-116. https://doi.org/10.1189/jlb.0809566
2. Morise J, Takematsu H, Oka S. The role of human natural killer-1 (HNK-1) carbohydrate in neuronal plasticity and disease. Biochim Biophys Acta Gen Subj. 2017;1861(10):2455-2461. https://doi.org/10.1016/j.bbagen.2017.06.025
3. Nielsen CM, White MJ, Goodier MR, Riley EM. Functional Significance of CD57 Expression on Human NK Cells and Relevance to Disease. Front Immunol. 2013;4:422. Published 2013 Dec 9. https://doi.org/10.3389/fimmu.2013.00422
4. Lian J, Yue Y, Yu W, Zhang Y. Immunosenescence: a key player in cancer development. J Hematol Oncol. 2020;13(1):151. Published 2020 Nov 10. https://doi.org/10.1186/s13045-020-00986-z6
Alternate Names
HNK1, HNK-1, Human Natural Killer-1, L2, LEU7, LEU-7
Gene Symbol
B3GAT1
UniProt
Additional CD57 Products
Product Documents for CD57 Antibody (SPM129)
Product Specific Notices for CD57 Antibody (SPM129)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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