CD68/SR-D1 Antibody (C68/684)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32832
![Flow Cytometry: CD68/SR-D1 Antibody (C68/684) [NBP2-32832]](https://resources.bio-techne.com/images/products/CD68-SR-D1-Antibody-C68-684-Flow-Cytometry-NBP2-32832-img0008.jpg "Flow Cytometry: CD68/SR-D1 Antibody (C68/684) [NBP2-32832]")
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (C68/684) [NBP2-32832]](https://resources.bio-techne.com/images/products/CD68-SR-D1-Antibody-C68-684-Immunohistochemistry-Paraffin-NBP2-32832-img0005.jpg "Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (C68/684) [NBP2-32832]")
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (C68/684) [NBP2-32832]](https://resources.bio-techne.com/images/products/CD68-SR-D1-Antibody-C68-684-Immunohistochemistry-Paraffin-NBP2-32832-img0006.jpg "Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (C68/684) [NBP2-32832]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Monkey
Applications
Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # C68/684
Concentration
0.2 mg/ml
Product Summary for CD68/SR-D1 Antibody (C68/684)
Immunogen
Recombinant human CD68/SR-D1 protein (Uniprot: P34810)
Localization
Cell Surface and Cytoplasmic
Specificity
This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocyticneoplasia. Lymphomas are negative or show few granules.
Marker
Macrophage Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34587)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD68/SR-D1 Antibody (C68/684)
Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (C68/684) [NBP2-32832]
Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (C68/684) [NBP2-32832] - Formalin-fixed, paraffin-embedded human Tonsil stained with .![Western Blot: CD68/SR-D1 Antibody (C68/684) [NBP2-32832]](https://resources.bio-techne.com/images/products/CD68-SR-D1-Antibody-C68-684-Western-Blot-NBP2-32832-img0007.jpg "Western Blot: CD68/SR-D1 Antibody (C68/684) [NBP2-32832]")
Western Blot: CD68/SR-D1 Antibody (C68/684) [NBP2-32832]
Western Blot: CD68/SR-D1 Antibody (C68/684) [NBP2-32832] - Western Blot Analysis of human Spleen tissue lysate using CD68/SR-D1 Antibody (C68/684).![Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (C68/684) [NBP2-32832]](https://resources.bio-techne.com/images/products/CD68-SR-D1-Antibody-C68-684-Immunocytochemistry-Immunofluorescence-NBP2-32832-img0004.jpg "Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (C68/684) [NBP2-32832]")
Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (C68/684) [NBP2-32832]
Immunocytochemistry/Immunofluorescence: CD68/SR-D1 Antibody (C68/684) [NBP2-32832] - Immunofluorescence staining of U87MG cells using CD68/SR-D1 Antibody (C68/684) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red DotApplications for CD68/SR-D1 Antibody (C68/684)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD68/SR-D1
CD68 is highly expressed in cells of the mononuclear phagocyte system such as macrophages, microglia, osteoclasts, and myeloid dendritic cells (DCs); and is expressed to a lesser extent in lymphoid cells (CD19+ B lymphocytes and CD4+ T lymphocytes), human umbilical cord mesenchymal stem cells (MSCs), fibroblasts, endothelial cells, multiple non-hematopoietic cancer cell lines, and human arterial intimal smooth muscle cells (SMCs). Expression has been also observed in diseased states for granulocytes and neutrophils, in particular basophils from myeloproliferative disorders and intestinal neutrophils from inflammatory bowel disease (IBD), respectively (1).
Although the function of CD68 has yet to be established, it has often been used as an immunohistochemistry (IHC) marker of inflammation and for granular cell tumors (GCTs). CD68+ tumor associated macrophages (TAMs) has been suggested to be a predictive marker for poor cancer prognosis, but a meta-analysis showed the presence of CD68 is not correlated with survival (2). In addition, a role in hepatic malaria infection has been reported based on the finding that peptide P39 binds CD68, considered a receptor for malaria sporozoite, and inhibits parasite entry into Kupffer cells. CD68 was deemed a member of the Scavenger receptor family due to its upregulation in macrophages following inflammatory stimuli, ability to bind modified LDL, phosphatidylserine, and apoptotic cells, as well as shuttling between the plasma membrane and endosomes. CD68 has been linked to atherogenesis based on binding and internalization of its ligand, oxLDL (1).
References
1. Chistiakov, DA, Killingsworth, MC, Myasoedova, VA. Orekhov AN, Bobryshev YV. (2017) CD68/macrosialin: not just a histochemical marker. Lab Invest. 97:4-13. PMID: 27869795
2. Troiano G, Caponio VCA, Adipietro I, Tepedino M, Santoro R, Laino L, Lo Russo L, Cirillo N, Lo Muzio L. (2019) Prognostic significance of CD68+ and CD163+ tumor associated macrophages in head and neck squamous cell carcinoma: A systematic review and meta-analysis. Oral Oncol. 93:66-75. PMID: 31109698.
Additional CD68/SR-D1 Products
Product Documents for CD68/SR-D1 Antibody (C68/684)
Product Specific Notices for CD68/SR-D1 Antibody (C68/684)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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