Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1E6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all CD68/SR-D1 Antibodies »

Product Specifications

Immunogen

This CD68/SR-D1 Antibody (OTI1E6) was developed against human recombinant protein fragment corresponding to amino acids 22-319 of human CD68 (NP_001242)produced in SF9 cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free

Western Blot: CD68/SR-D1 Antibody (OTI1E6)Azide and BSA Free [NBP2-70381]

Western Blot: CD68/SR-D1 Antibody (OTI1E6)Azide and BSA Free [NBP2-70381]

Western Blot: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD68.
Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381]

Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381]

Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381] - Analysis of Human lymphoma tissue.
Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381]

Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381]

Immunohistochemistry: CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free [NBP2-70381] - Analysis of Adenocarcinoma of Human breast tissue.
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Applications for CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD68/SR-D1

Human CD68, also known as GP110, LAMP4, Scavenger Receptor D1 (SR-D1) or macrosialin in mouse, encodes a 110-kD transmembrane glycoprotein that belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. Members of the LAMP family include LAMP-1, LAMP-2, dendritic cell (DC)-LAMP (aka CD208), and brain and dendritic cell-associated (BAD)-LAMP (aka LAMP-5). Unlike the two LAMP domains facing the lysosomal lumen in LAMP-1 and LAMP-2, CD68 has a single LAMP domain containing four cystines spaced 36-37 residues apart along with an N-terminal Mucin-like domain. The 354 amino acid (a.a.) human CD68 and 326 a.a. murine ortholog share 80.6% a.a. sequence identity (1).

CD68 is highly expressed in cells of the mononuclear phagocyte system such as macrophages, microglia, osteoclasts, and myeloid dendritic cells (DCs); and is expressed to a lesser extent in lymphoid cells (CD19+ B lymphocytes and CD4+ T lymphocytes), human umbilical cord mesenchymal stem cells (MSCs), fibroblasts, endothelial cells, multiple non-hematopoietic cancer cell lines, and human arterial intimal smooth muscle cells (SMCs). Expression has been also observed in diseased states for granulocytes and neutrophils, in particular basophils from myeloproliferative disorders and intestinal neutrophils from inflammatory bowel disease (IBD), respectively (1).

Although the function of CD68 has yet to be established, it has often been used as an immunohistochemistry (IHC) marker of inflammation and for granular cell tumors (GCTs). CD68+ tumor associated macrophages (TAMs) has been suggested to be a predictive marker for poor cancer prognosis, but a meta-analysis showed the presence of CD68 is not correlated with survival (2). In addition, a role in hepatic malaria infection has been reported based on the finding that peptide P39 binds CD68, considered a receptor for malaria sporozoite, and inhibits parasite entry into Kupffer cells. CD68 was deemed a member of the Scavenger receptor family due to its upregulation in macrophages following inflammatory stimuli, ability to bind modified LDL, phosphatidylserine, and apoptotic cells, as well as shuttling between the plasma membrane and endosomes. CD68 has been linked to atherogenesis based on binding and internalization of its ligand, oxLDL (1).

References

1. Chistiakov, DA, Killingsworth, MC, Myasoedova, VA. Orekhov AN, Bobryshev YV. (2017) CD68/macrosialin: not just a histochemical marker. Lab Invest. 97:4-13. PMID: 27869795

2. Troiano G, Caponio VCA, Adipietro I, Tepedino M, Santoro R, Laino L, Lo Russo L, Cirillo N, Lo Muzio L. (2019) Prognostic significance of CD68+ and CD163+ tumor associated macrophages in head and neck squamous cell carcinoma: A systematic review and meta-analysis. Oral Oncol. 93:66-75. PMID: 31109698.

Alternate Names

CD68, gp110, Macrosialin, SCARD1, SR-D1, SRD1

Gene Symbol

CD68

Additional CD68/SR-D1 Products

Product Documents for CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD68/SR-D1 Antibody (OTI1E6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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