CD8 Antibody (BLR044F)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76422

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-76422
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR044F

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD8 Antibodies »

Product Summary for CD8 Antibody (BLR044F)

Immunogen

Recombinant monoclonal CD8 Antibody (BLR044F) was developed against an immunogen sequence between amino acids 185 and 235 (C-Term)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD8 Antibody (BLR044F)

Western Blot: CD8 Antibody (BLR044F) [NBP2-76422]

Western Blot: CD8 Antibody (BLR044F) [NBP2-76422]

Western Blot: CD8 Antibody (BLR044F) [NBP2-76422] - Detection of human CD8 by WB of CD4+ T-cells, CD8+ T-cells, Jurkat, MOLT-4, RPMI-8226, MJ, C5/MJ, KG-1, SR, and K-562 cells. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal [BLR044F] (NBP2-76422). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower panel: Recombinant monoclonal antibodies to CD3e (NBP2-76424) and CD4 (NBP2-76423). A band appears at an observed molecular weight of approximately 32 kDa for the CD8 analysis.
Immunocytochemistry/ Immunofluorescence: CD8 Antibody (BLR044F) [NBP2-76422]

Immunocytochemistry/ Immunofluorescence: CD8 Antibody (BLR044F) [NBP2-76422]

Immunocytochemistry/Immunofluorescence: CD8 Antibody (BLR044F) [NBP2-76422] - Multiplex immunofluorescence analysis using rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] , rabbit anti-CD8 alpha recombinant monoclonal [BLR044F], rabbit anti-PD-L1 recombinant monoclonal [BLR020E]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™ 520, 620, and 690. Counterstain: DAPI (blue).
Immunohistochemistry-Paraffin: CD8 Antibody (BLR044F) [NBP2-76422]

Immunohistochemistry-Paraffin: CD8 Antibody (BLR044F) [NBP2-76422]

Immunohistochemistry-Paraffin: CD8 Antibody (BLR044F) [NBP2-76422] - Detection of human CD8 alpha in FFPE tonsil by IHC. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal [BLR044F] (NBP2-76422). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
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Applications for CD8 Antibody (BLR044F)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. Recommended dilution for multiplex immunofluorescence: 1:250
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: CD8

CD8, also known as Leu-2 or T8 in human and Lyt2 or Lyt3 in mouse, is a cell surface glycoprotein belonging to the immunoglobulin supergene family (1, 2). CD8 is expressed on cytotoxic T-lymphocytes (T-cells), most thymocytes, between 35-45% of peripheral blood lymphocytes, and a population of natural killer (NK) cells (1, 2). The CD8 molecule consists of disulfide-linked alpha (alpha) and beta (beta) chains that present on T-cells as either CD8alphaalpha homodimers or CD8alphabeta heterodimers (1, 3). Both alpha and beta chains consist of a signaling sequence, an extracellular Ig-like domain, a membrane proximal stalk region, a transmembrane domain, and a cytoplasmic tail (3). Human CD8alpha is processed as 235 amino acids (aa) in length with a theoretical molecular weight of ~26 kDa, while mouse CD8alpha is 247 aa and has a theoretical molecular weight of 27.5 kDa (4, 5). Functionally, CD8 acts as an antigen coreceptor on cytotoxic T-cells and interacts with the major histocompatibility complex (MHC) class I molecules on antigen presenting cells (APCs), mediating cell-cell interactions within the immune system. Conversely, CD4 molecules interact with antigens presented on MHC class II molecules and are activated to become helper T-cells (TH) (1,2). Interestingly, thymocytes can transiently express both CD4 and CD8 during the maturation process (2). Furthermore, the cytoplasmic tail of CD8 has a Lck (lymphocyte-specific protein tyrosine kinase) binding domain where Lck interacts with CD8, initiating a phosphorylation cascade that activates transcription factors and promotes T-cell activation (6). More specifically, CD8alphabeta functions as a T-cell co-receptor, while CD8alphaalpha promotes T-cell survival and differentiation (7).

Given its role in the immune system, CD8-deficiency in T-cells is a hallmark of many diseases and pathologies (8-10). Specifically, CD8+ T-cell deficiency is prevalent in chronic autoimmune diseases including multiple sclerosis, rheumatoid arthritis, ulcerative colitis, Crohn's disease, type 1 diabetes mellitus, and Graves' disease (8). Furthermore, cancers or chronic infection can lead to CD8 T-cell exhaustion as the continual antigen presentation and inflammatory signals eventually cause the CD8+ T-cells to lose functionality (9, 10). However, animal models and clinical studies have suggested that T-cells are capable of being reinvigorated using inhibitory receptor blockade resulting in better disease outcomes and these exhausted T-cells may be a potential therapeutic target (9, 10).

Alternative names for CD8 includes CD antigen: CD8a, CD8 antigen, alpha polypeptide (p32), CD8a molecule, CD8A, Leu2 T-lymphocyte antigen, LEU2, MAL, OKT8 T-cell antigen, p32, T cell co-receptor, T8 T-cell antigen, T-cell antigen Leu2, T-cell surface glycoprotein CD8 alpha chain, and T-lymphocyte differentiation antigen T8/Leu-2.

References

1. Littman D. R. (1987). The structure of the CD4 and CD8 genes. Annual review of immunology. https://doi.org/10.1146/annurev.iy.05.040187.003021

2. Naeim F. (2008). Chapter 2- Principles of Immunophenotyping. Hematopathology. https://doi.org/10.1016/B978-0-12-370607-2.00002-8.

3. Gao, G. F., & Jakobsen, B. K. (2000). Molecular interactions of coreceptor CD8 and MHC class I: the molecular basis for functional coordination with the T-cell receptor. Immunology today. https://doi.org/10.1016/s0167-5699(00)01750-3

4. UniProt (P01732)

5. UniProt (P01731)

6. Kappes D. J. (2007). CD4 and CD8: hogging all the Lck. Immunity. https://doi.org/10.1016/j.immuni.2007.11.002

7. Gangadharan, D., & Cheroutre, H. (2004). The CD8 isoform CD8alphaalpha is not a functional homologue of the TCR co-receptor CD8alphabeta. Current opinion in immunology. https://doi.org/10.1016/j.coi.2004.03.015

8. Pender M. P. (2012). CD8+ T-Cell Deficiency, Epstein-Barr Virus Infection, Vitamin D Deficiency, and Steps to Autoimmunity: A Unifying Hypothesis. Autoimmune diseases. https://doi.org/10.1155/2012/189096

9. Kurachi M. (2019). CD8+ T cell exhaustion. Seminars in immunopathology. https://doi.org/10.1007/s00281-019-00744-5

10. Hashimoto, M., Kamphorst, A. O., Im, S. J., Kissick, H. T., Pillai, R. N., Ramalingam, S. S., Araki, K., & Ahmed, R. (2018). CD8 T Cell Exhaustion in Chronic Infection and Cancer: Opportunities for Interventions. Annual review of medicine. https://doi.org/10.1146/annurev-med-012017-043208

Alternate Names

CD8, CD8A

Gene Symbol

CD8A

Additional CD8 Products

Product Documents for CD8 Antibody (BLR044F)

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Product Specific Notices for CD8 Antibody (BLR044F)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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