CD81 Antibody (1D6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65805

Clone 1D6 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB100-65805
NB100-65805SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Goat, Primate, Sheep

Cited:

Human, Primate

Applications

Validated:

CyTOF-ready, Dot Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Cytometric Bead Assay Standard, ELISA, Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D6

Format

BSA Free

Concentration

1.0 mg/ml

View all CD81 Antibodies »

Product Specifications

Immunogen

Aggregated OCI-Ly8 human B cell line

Reactivity Notes

Mouse reactivity reported in scientific literature (Ji et al). Canine reactivity reported from a verified customer review. Primate reactivity reported in scientific literature (PMID:33077711).

Localization

Membrane; Multi-pass membrane protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CD81 Antibody (1D6) - BSA Free

Western Blot: CD81 Antibody (1D6)BSA Free [NB100-65805]

Western Blot: CD81 Antibody (1D6)BSA Free [NB100-65805]

Western Blot: CD81 Antibody (1D6) [NB100-65805] - Isolation and characterization of exosomes from cell culture supernatant and human blood. Immunoblot of exosomal markers CD81 (~30 kDa), CD63 (~55 kDa), and LAMP2B (~50 kDa) in exosomes (5 ug lysates) isolated from the serum of breast cancer patients and healthy control. Grp94 (~100 kDa) and beta-actin (~42 kDa) serve as a negative control and a loading control, respectively. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep36502), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: CD81 Antibody (1D6) - BSA Free [NB100-65805]

Immunohistochemistry-Paraffin: CD81 Antibody (1D6) - BSA Free [NB100-65805]

Immunohistochemistry-Paraffin: CD81 Antibody (1D6) [NB100-65805] - Staining of TAPA1 in human testis using DAB with hematoxylin counterstain.
Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805]

Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805]

Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 Antibody (1D6) NB100-65805 (blue) and a matched isotype control MAB002 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).
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Applications for CD81 Antibody (1D6) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.2 - 1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:10 - 1:100

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:10 - 1:500

Western Blot

1 - 2 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB100-65805 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD81

TAPA1, also known as CD81, is found in the plasma membrane in lymphocytes and is believed to play an important role in the regulation of lymphoma cell growth. This protein is primarily found on B and T cells (lymphocytes) in humans. TAPA1 appears to be involved in a variety of cellular processes including activation, proliferation and differentiation. It is believed to act as a viral receptor for HCV and may play a crucial role in cell signaling from its place embedded in the plasma membrane. Mutations affecting CD81 can cause immunodeficiency- a disease that is characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections due to a lack of an immune response.

Alternate Names

CD81, TAPA-1, TSPAN28

Gene Symbol

CD81

Additional CD81 Products

Product Documents for CD81 Antibody (1D6) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD81 Antibody (1D6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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