CD82/Kai-1 Antibody (0T3L6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16797
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 0T3L6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 168-267 of human CD82/Kai-1 (P27701). PEVTYPCSCEVKGEEDNSLSVRKGFCEAPGNRTQSGNHPEDWPVYQEGCMEKVQAWLQENLGIILGVGVGVAIIELLGMVLSICLCRHVHSEDYSKVPKY
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CD82/Kai-1 Antibody (0T3L6)
![Western Blot: CD82/Kai-1 Antibody (0T3L6) [NBP3-16797]](https://resources.bio-techne.com/images/products/CD82-Kai-1-Antibody-0T3L6-Western-Blot-NBP3-16797-img0004.jpg "Western Blot: CD82/Kai-1 Antibody (0T3L6) [NBP3-16797]")
Western Blot: CD82/Kai-1 Antibody (0T3L6) [NBP3-16797]
Western Blot: CD82/Kai-1 Antibody (0T3L6) [NBP3-16797] - Analysis of extracts of various cell lines, using CD82/Kai-1 Rabbit mAb (NBP3-16797) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody (0T3L6) [NBP3-16797] -
Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody (0T3L6) [NBP3-16797] -Human tonsil tissue using CD82 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunocytochemistry/Immunofluorescence: CD82/Kai-1 Antibody (0T3L6) [NBP3-16797] -
Immunocytochemistry/Immunofluorescence: CD82/Kai-1 Antibody (0T3L6) [NBP3-16797] - Confocal imaging of RAW 264.7 cells using CD82 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.Applications for CD82/Kai-1 Antibody (0T3L6)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:200 - 1:2000
Immunohistochemistry
1:100 - 1:500
Immunohistochemistry-Paraffin
1:100 - 1:500
Western Blot
1:1000 - 1:6000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CD82/Kai-1
Alternate Names
CD82, IA4, KAI1, SAR2, ST6, TSPAN27
Gene Symbol
CD82
Additional CD82/Kai-1 Products
Product Documents for CD82/Kai-1 Antibody (0T3L6)
Product Specific Notices for CD82/Kai-1 Antibody (0T3L6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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