CD82/Kai-1 Antibody (0T3L6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16797
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 0T3L6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for CD82/Kai-1 Antibody (0T3L6)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 168-267 of human CD82/Kai-1 (P27701). PEVTYPCSCEVKGEEDNSLSVRKGFCEAPGNRTQSGNHPEDWPVYQEGCMEKVQAWLQENLGIILGVGVGVAIIELLGMVLSICLCRHVHSEDYSKVPKY
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CD82/Kai-1 Antibody (0T3L6)
Western Blot: CD82/Kai-1 Antibody (0T3L6) [NBP3-16797]
Western Blot: CD82/Kai-1 Antibody (0T3L6) [NBP3-16797] - Analysis of extracts of various cell lines, using CD82/Kai-1 Rabbit mAb (NBP3-16797) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Immunocytochemistry/ Immunofluorescence: CD82/Kai-1 Antibody (0T3L6) [NBP3-16797]
Immunocytochemistry/Immunofluorescence: CD82/Kai-1 Antibody (0T3L6) [NBP3-16797] - Immunofluorescence analysis of Raw264 7 cells using CD82/Kai-1 Rabbit mAb (NBP3-16797) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody (0T3L6) [NBP3-16797]
Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody (0T3L6) [NBP3-16797] - Human colon using CD82/Kai-1 Rabbit mAb (NBP3-16797) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for CD82/Kai-1 Antibody (0T3L6)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CD82/Kai-1
Alternate Names
CD82, IA4, KAI1, SAR2, ST6, TSPAN27
Gene Symbol
CD82
Additional CD82/Kai-1 Products
Product Documents for CD82/Kai-1 Antibody (0T3L6)
Product Specific Notices for CD82/Kai-1 Antibody (0T3L6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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