Skip to main content

CD84/SLAMF5 Antibody (153-4D9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44346

Clone 153-4D9 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP2-44346-0.02mg
NBP2-44346-0.1mg

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 153-4D9

Concentration

0.2 mg/ml

Product Specifications

Immunogen

MLR-stimulated lymphocytes

Localization

Cell Surface

Specificity

Recognizes a protein of 74kDa, identified as CD84. It is expressed on mature B cells and on B-cell lines, including pre-B-cell lines, but not on plasma cell lines. Immunohistochemical studies demonstrated that CD84 strongly expressed on tissues macrophages. CD84 is also highly expressed on platelets and, at low levels on peripheral blood T cells. It is a highly N-glycosylated protein and belongs to immunoglobulin superfamily. It may play a role in leukocyte activation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11578)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD84/SLAMF5 Antibody (153-4D9)

Flow Cytometry: CD84/SLAMF5 Antibody (153-4D9) [NBP2-44346]

Flow Cytometry: CD84/SLAMF5 Antibody (153-4D9) [NBP2-44346]

Flow Cytometry: CD84/SLAMF5 Antibody (153-4D9) [NBP2-44346] - Flow Cytometric Analysis of PFA-fixed MOLT4 cells. CD84/SLAMF5 Antibody (153-4D9) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Applications for CD84/SLAMF5 Antibody (153-4D9)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD84/SLAMF5

CD84 is a 64-82 kD glycoprotein. It is a member of the SLAM (CD150) family, a CD2 subset of the Ig superfamily, also known as SLAMF5 or Ly9b. CD84 is expressed on B cells, monocytes, thymocytes, subset of T cells, and platelets. CD84 functions as a homophilic adhesion molecule and enhances T cell activation and cytokine production. CD84 is expressed on mature B cells and B cell lines but not on plasma cell lines. Immunohistochemical studies demonstrate that it strongly stains tissue macrophages. It is also expressed on platelets and at low levels on blood T cells. Cellular expression does not significantly increase after activation.

Long Name

SLAM Family Member 5

Alternate Names

CD84, Ly-9B, SLAMF5

Gene Symbol

CD84

Additional CD84/SLAMF5 Products

Product Documents for CD84/SLAMF5 Antibody (153-4D9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD84/SLAMF5 Antibody (153-4D9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...
Loading...