CD9 Antibody (SN4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43444

Clone SN4 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP1-43444-0.1mg
NBP1-43444-25ug
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # SN4

Format

BSA Free

Concentration

0.5 mg/ml

View all CD9 Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD9.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD9 Antibody (SN4) - BSA Free

Flow Cytometry: CD9 Antibody (SN4) - BSA Free [NBP1-43444]

Flow Cytometry: CD9 Antibody (SN4) - BSA Free [NBP1-43444]

Flow Cytometry: CD9 Antibody (SN4) [NBP1-43444] - Analysis using the FITC conjugate of NBP1-43444. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control FITC (blue histogram) or Anti-Human CD9 FITC (purple histogram). Cells in the lymphocyte (left) or monocyte (right) gates were used for analysis.
Flow Cytometry: CD9 Antibody (SN4) - BSA Free [NBP1-43444]

Flow Cytometry: CD9 Antibody (SN4) - BSA Free [NBP1-43444]

Flow Cytometry: CD9 Antibody (SN4) [NBP1-43444] - Staining of normal human peripheral blood cells with 0.25 ug of Mouse IgG11 K Isotype Control Purified (open histogram) or 0.25 ug of Anti-Human CD9 Purified (filled histogram) followed by Anti-Mouse IgG FITC . Total viable cells were used for analysis.

Applications for CD9 Antibody (SN4) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
This SN4 antibody has been tested by flow cytometric analysis of normal human platelets. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD9

The SN4 monoclonal antibody reacts with human CD9. CD9 is a 24 kDa member of the tetraspanin family, whose members are characterized by the presence of 4 hydrophobic transmembrane domains. CD9 is expressed in platelets, eosinophils, basophils, pre-B cells, activated T cells and neural cell lines. Furthermore, CD9 expression has been associated with a malignant phenotype, including expression on 90% of non T cell acute lymphoblastic leukemia cells and on 50% of chronic lymphocytic and acute myeloblastic leukemias. In platelets, CD9 is expressed in alpha-granules and through association with the integrin alphaIIb/betaIII plays a role in platelet aggregation, as demonstrated by the use of anti-human CD9 antibodies. CD9 has also been shown to induce the aggregation of pre-B cell lines, and the adhesion and migration of pre-B cells and Schwann cells. Additionally, it has been demonstrated that CD9 is able to provide a co-stimulatory signal for T cells independently of CD28, in the absence of antigen-presenting cells. Binding of the SN4 monoclonal antibody partially cross-blocks binding of another anti-human CD9 monoclonal antibody, MM2/57.

Alternate Names

CD9, DRAP-27, MIC3, p24, TSPAN29

Gene Symbol

CD9

Additional CD9 Products

Product Documents for CD9 Antibody (SN4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD9 Antibody (SN4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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