CD96 Antibody (6A6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11254

Novus Biologicals, part of Bio-Techne
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NBP3-11254
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Functional, Immunoassay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 6A6

Concentration

0.1 mg/ml

View all CD96 Antibodies »

Product Specifications

Immunogen

The monoclonal antibody 6A6 was raised after immunization of rats with purified recombinant mCD96-hIgG1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<24 EU/mg

Applications for CD96 Antibody (6A6)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Western blotting and Flow Cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies dilutions have to be made according to the amounts of CD96 to be inactivated.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD96

The monoclonal antibody 6A6 recognizes mouse CD96. CD96 or T cell-Activated Increased Late Expression (TACTILE) is an ~67 kD immunoglobulin-like protein expressed on natural killer (NK) and T cells. CD96 belongs to the Ig-super family, consisting of three Ig-like domains and an extended serine/threonine/praline-rich stalk in the ectodomain. CD96 is upregulated upon T cell activation and is involved in engagement of diseased cells and cell movement towards areas of inflammation. Mouse CD96 binds to CD155 and nectin-1. CD96/CD155-interaction is preserved between man and mouse although both receptors are only moderately conserved in amino acid sequence. The monoclonal antibody 6A6 completely inhibits binding of CD96 to both recombinant mouse CD155 and mouse nectin-1. Also, T cell adhesion to insect cells expressing mCD155 is blocked by this monoclonal antibody, however, depending on the T cell subtype. The monoclonal antibody 6A6 was raised after immunization of rats with purified recombinant mCD96-hIgG1.

Alternate Names

CD96, TACTILE

Gene Symbol

CD96

Additional CD96 Products

Product Documents for CD96 Antibody (6A6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD96 Antibody (6A6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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