CD97 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99765

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD97 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human CD97 (Accession#: NP_001775.2; Met1-Gln398)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD97 Antibody

Western Blot: CD97 Antibody [NBP2-99765]

Western Blot: CD97 Antibody [NBP2-99765]

Western Blot: CD97 Antibody [NBP2-99765] - Anti-CD97 rabbit polyclonal antibody at 1:500 dilution. Lane A: HL-60 Membrane Lysate. Lane B: K562 Membrane Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 70 kDa
Immunocytochemistry/ Immunofluorescence: CD97 Antibody [NBP2-99765]

Immunocytochemistry/ Immunofluorescence: CD97 Antibody [NBP2-99765]

Immunocytochemistry/Immunofluorescence: CD97 Antibody [NBP2-99765] - Immunofluorescence staining of CD97 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human CD97 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.
Immunoprecipitation: CD97 Antibody [NBP2-99765]

Immunoprecipitation: CD97 Antibody [NBP2-99765]

Immunoprecipitation: CD97 Antibody [NBP2-99765] - CD97 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate 2 ul anti-CD97 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-CD97 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 92 kDa. Observed band size: 92 kDa

Applications for CD97 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunoprecipitation

0.5-2 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD97

CD97, a CD55 Receptor, is a leukocyte antigen located on most activated leukocytes. CD97 is highly homologous to EMR2 with five EGF-like domains in the N-terminal extracellular domain, suggesting that it may be involved in cell signaling as well as in cell adhesion (Hamann et al., 1996). Two variants are produced by alternative splicing, where the shorter form lacks exons 5 and 6 and reduces the number of EGF-like domains from 5 to 3, compared to the full-length isoform. CD97 is associated with with the activated lesions of multiple sclerosis. CD97 expression is also implicated in the dedifferentiation of colon and thyroid tumors. CD97 is expressed abundantly in cells of hematopoietic origin and is markedly upregulated on activated T and B cells. ESTs have been isolated from a wide variety of tissue libraries.

Alternate Names

CD97

Gene Symbol

ADGRE5

Additional CD97 Products

Product Documents for CD97 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD97 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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