CD99 Antibody (HO36-1.1) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47894
Key Product Details
Species Reactivity
Human, Rat
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Kappa Clone # HO36-1.1
Format
Azide and BSA Free
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Purified E-rosette forming cells from human peripheral blood lymphocytes
Localization
Cell surface
Specificity
Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32).Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewings sarcoma cells and primitive peripheral neuroectodermal tumors. This MAb shows a very similar reactivity to other CD99 MAbs (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Marker
Ewing's Sarcoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgM Kappa
Description
Purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS WITHOUT BSA & azide at 1.0 mg/ml.
Scientific Data Images for CD99 Antibody (HO36-1.1) - Azide and BSA Free
Immunohistochemistry-Paraffin: CD99 Antibody (HO36-1.1) - Azide and BSA Free [NBP2-47894]
Immunohistochemistry-Paraffin: CD99 Antibody (HO36-1.1) - Azide and BSA Free [NBP2-47894] - Human Ewing's Sarcoma stained with CD99 Monoclonal Antibody (HO36-1.1).Immunohistochemistry-Paraffin: CD99 Antibody (HO36-1.1) - Azide and BSA Free [NBP2-47894]
Immunohistochemistry-Paraffin: CD99 Antibody (HO36-1.1) - Azide and BSA Free [NBP2-47894] - Human Testicular Carcinoma stained with CD99 Monoclonal Antibody (HO36-1.1).Applications for CD99 Antibody (HO36-1.1) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
5 - 10 ul/million cells in 0.1 ml
Immunocytochemistry/ Immunofluorescence
0.5-1 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
1 - 2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein L purified
Formulation
10 mM PBS with 0.05% BSA
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD99
Additional CD99 Products
Product Documents for CD99 Antibody (HO36-1.1) - Azide and BSA Free
Product Specific Notices for CD99 Antibody (HO36-1.1) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...