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Cdc14A Antibody (2C12)

Novus Biologicals, part of Bio-Techne | Catalog # H00008556-M01

Novus Biologicals, part of Bio-Techne
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H00008556-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2C12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

CDC14A (NP_003663, 431 a.a. ~ 530 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PFRLSSSLQGSAVTLKTSKMALSPSATAKRINRTSLSSGATVRSFSINSRLASSLGNLNAATDDPENKKTSSSSKAGFTASPFTNLLNGSSQPTTRNYPE

Specificity

CDC14A - CDC14 cell division cycle 14 homolog A (S. cerevisiae)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Cdc14A Antibody (2C12)

Western Blot: Cdc14A Antibody (2C12) [H00008556-M01]

Western Blot: Cdc14A Antibody (2C12) [H00008556-M01]

Western Blot: Cdc14A Antibody (2C12) [H00008556-M01] - Analysis of CDC14A expression in transfected 293T cell line by CDC14A monoclonal antibody (M01), clone 2C12.Lane 1: CDC14A transfected lysate(66.6 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Cdc14A Antibody (2C12) [H00008556-M01]

Immunocytochemistry/ Immunofluorescence: Cdc14A Antibody (2C12) [H00008556-M01]

Immunocytochemistry/Immunofluorescence: Cdc14A Antibody (2C12) [H00008556-M01] - Analysis of monoclonal antibody to CDC14A on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: Cdc14A Antibody (2C12) [H00008556-M01] - Detection limit for recombinant GST tagged CDC14A is approximately 3ng/ml as a capture antibody.

Applications for Cdc14A Antibody (2C12)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cdc14A

The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. It is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, suggesting a role in cell cycle control. This protein has been shown to interact with, and dephosphorylate tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splicing of this gene results in several transcript variants encoding distinct isoforms.

Alternate Names

CDC10 (cell division cycle 10, S. cerevisiae, homolog), cdc14, CDC14 cell division cycle 14 homolog A, CDC14 cell division cycle 14 homolog A (S. cerevisiae), Cdc14A1, Cdc14A2, dual specificity protein phosphatase CDC14A, EC 3.1.3.16, EC 3.1.3.48, hCDC14

Entrez Gene IDs

8556 (Human)

Gene Symbol

CDC14A

OMIM

603504 (Human)

Additional Cdc14A Products

Product Documents for Cdc14A Antibody (2C12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cdc14A Antibody (2C12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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