Cdc14A Antibody (2C12)
Novus Biologicals, part of Bio-Techne | Catalog # H00008556-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 2C12
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
CDC14A (NP_003663, 431 a.a. ~ 530 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PFRLSSSLQGSAVTLKTSKMALSPSATAKRINRTSLSSGATVRSFSINSRLASSLGNLNAATDDPENKKTSSSSKAGFTASPFTNLLNGSSQPTTRNYPE
Specificity
CDC14A - CDC14 cell division cycle 14 homolog A (S. cerevisiae)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Cdc14A Antibody (2C12)
Western Blot: Cdc14A Antibody (2C12) [H00008556-M01]
Western Blot: Cdc14A Antibody (2C12) [H00008556-M01] - Analysis of CDC14A expression in transfected 293T cell line by CDC14A monoclonal antibody (M01), clone 2C12.Lane 1: CDC14A transfected lysate(66.6 KDa).Lane 2: Non-transfected lysate.Immunocytochemistry/ Immunofluorescence: Cdc14A Antibody (2C12) [H00008556-M01]
Immunocytochemistry/Immunofluorescence: Cdc14A Antibody (2C12) [H00008556-M01] - Analysis of monoclonal antibody to CDC14A on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: Cdc14A Antibody (2C12) [H00008556-M01] - Detection limit for recombinant GST tagged CDC14A is approximately 3ng/ml as a capture antibody.
Applications for Cdc14A Antibody (2C12)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Cdc14A
Alternate Names
CDC10 (cell division cycle 10, S. cerevisiae, homolog), cdc14, CDC14 cell division cycle 14 homolog A, CDC14 cell division cycle 14 homolog A (S. cerevisiae), Cdc14A1, Cdc14A2, dual specificity protein phosphatase CDC14A, EC 3.1.3.16, EC 3.1.3.48, hCDC14
Entrez Gene IDs
8556 (Human)
Gene Symbol
CDC14A
OMIM
603504 (Human)
UniProt
Additional Cdc14A Products
Product Documents for Cdc14A Antibody (2C12)
Product Specific Notices for Cdc14A Antibody (2C12)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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