CDC16 [p Thr580] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-889
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This affinity purified CDC16 [p Thr580] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding pT580 of Human CDC16 protein. (Uniprot: Q13042)
Reactivity Notes
No cross reactivity is expected against homologues from other sources
Modification
p Thr580
Localization
Nuclear
Specificity
This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human CDC16 protein at the pT580 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with CDC16 from human. No cross reactivity is expected against homologues from other sources. This antibody is expected to react with all isoforms of this protein. Minimal reactivity is expected with the non-phosphorylated form of the protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for CDC16 [p Thr580] Antibody
Western Blot: CDC16 [p Thr580] Antibody [NB600-889] - Analysis of a band 72 kDa corresponding to phosphorylated human APC6 (arrowhead lane 1). Lane 1 - nocodazole treated HeLa whole cell lysate. Lane 2 - Reactivity is not seen in lysates from asynchronous HeLa whole cell cultures. Each lane contains approximately 35ug of lysates, separated by 4-20% SDS-PAGE Tris-HEPES and then transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000 overnight at 4C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
Applications for CDC16 [p Thr580] Antibody
Application
Recommended Usage
ELISA
1:1000-1:10000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Western Blot
1:500-1:3000
Application Notes
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 72 kDa, 71.5 kDa or 65.9 kDa in size corresponding to isoforms of APC6 by western blotting in the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: CDC16
Alternate Names
ANAPC6subunit 6, CDC16 (cell division cycle 16, S. cerevisiae, homolog), CDC16 cell division cycle 16 homolog (S. cerevisiae), CDC16 homolog, CDC16Hs, cell division cycle 16 homolog (S. cerevisiae), cell division cycle protein 16 homolog, Cyclosome subunit 6
Entrez Gene IDs
8881 (Human)
Gene Symbol
CDC16
UniProt
Additional CDC16 Products
Product Documents for CDC16 [p Thr580] Antibody
Product Specific Notices for CDC16 [p Thr580] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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