CDC2/CDK1 Antibody (BLR101H)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14693

Recombinant Monoclonal
Novus Biologicals, part of Bio-Techne
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NBP3-14693
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR101H

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CDC2/CDK1 Antibodies »

Product Specifications

Immunogen

surrounding tyrosine 15

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for CDC2/CDK1 Antibody (BLR101H)

Immunoprecipitation: CDC2/CDK1 Antibody (BLR101H) [NBP3-14693]

Immunoprecipitation: CDC2/CDK1 Antibody (BLR101H) [NBP3-14693]

Immunoprecipitation: CDC2/CDK1 Antibody [NBP3-14693] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with Hydroxyurea (+) or mock treated (-). Antibodies:Rabbit anti-Phospho CDK1 (Y15) recombinant monoclonalantibody used for IP at 20 ul/mg lysate. Total CDK1 was immunoprecipitated byRabbit anti-CDK1 recombinant monoclonal antibody. Immunoprecipitates where treatedwith phosphatase (+) or mock treated (-). For blottingimmunoprecipitated Phospho CDK1 (Y15), wasused at 1:1000. Chemiluminescence with an exposure timeof 10 seconds. Lower panel shows WB for total CDK1 usingrabbit anti-CDK1 recombinant monoclonal.

Applications for CDC2/CDK1 Antibody (BLR101H)

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: CDC2/CDK1

The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

Long Name

Cell Division Cycle 2/Cyclin-dependent Kinase 1

Alternate Names

CDK1

Gene Symbol

CDK1

Additional CDC2/CDK1 Products

Product Documents for CDC2/CDK1 Antibody (BLR101H)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CDC2/CDK1 Antibody (BLR101H)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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