Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CDC20/1102

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all Cdc20 Antibodies »

Product Summary for Cdc20 Antibody (CDC20/1102) - Azide and BSA Free

Immunogen

Recombinant human Cdc20 protein (Uniprot: Q12834)

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44407).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cdc20 Antibody (CDC20/1102) - Azide and BSA Free

Immunohistochemistry-Paraffin: Cdc20 Antibody (CDC20/1102) - Azide and BSA Free [NBP2-47811]

Immunohistochemistry-Paraffin: Cdc20 Antibody (CDC20/1102) - Azide and BSA Free [NBP2-47811]

Immunohistochemistry-Paraffin: Cdc20 Antibody (CDC20/1102) - Azide and BSA Free [NBP2-47811] - Human Gastric Carcinoma stained with CDC20 Monoclonal Antibody (CDC20/1102)
Immunohistochemistry-Paraffin: Cdc20 Antibody (CDC20/1102) - Azide and BSA Free [NBP2-47811]

Immunohistochemistry-Paraffin: Cdc20 Antibody (CDC20/1102) - Azide and BSA Free [NBP2-47811]

Immunohistochemistry-Paraffin: Cdc20 Antibody (CDC20/1102) - Azide and BSA Free [NBP2-47811] - Human Tonsil stained with CDC20 Monoclonal Antibody (CDC20/1102)

Applications for Cdc20 Antibody (CDC20/1102) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Cdc20

CDC20 is a WD repeat containing cell cycle-regulator that binds to and activates the ubiquitin-ligase activity of the APC/c (anaphase-promoting complex/cyclosome). APC/c ubiquitinating activity is responsible for the degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. CDC20 has also been reported to form a complex with histone deacetylases and can function as a transcriptional repressor involved in cell cycle arrest. As a regulator of APC/c activity, CDC20 plays a key role in the spindle-assembly checkpoint.

Long Name

Cell division cycle protein 20 homolog

Alternate Names

p55CDC

Gene Symbol

CDC20

Additional Cdc20 Products

Product Documents for Cdc20 Antibody (CDC20/1102) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cdc20 Antibody (CDC20/1102) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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