CDC25A Antibody (3D5)
Novus Biologicals, part of Bio-Techne | Catalog # H00000993-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3D5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
CDC25A (AAH07401, 151 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PVRPVSRGCLHSHGLQEGKDLFTQRQNSAPARMLSSNERDSSEPGNFIPLFTPQSPVTATLSDEDDGFVDLLDGENLKNEEETPSCMASLWTAPLVMRTT
Specificity
CDC25A - cell division cycle 25A
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for CDC25A Antibody (3D5)
![Proximity Ligation Assay: CDC25A Antibody (3D5) [H00000993-M01]](https://resources.bio-techne.com/images/products/CDC25A-Antibody-3D5-Proximity-Ligation-Assay-H00000993-M01-img0002.jpg "Proximity Ligation Assay: CDC25A Antibody (3D5) [H00000993-M01]")
Proximity Ligation Assay: CDC25A Antibody (3D5) [H00000993-M01]
Proximity Ligation Assay: CDC25A Antibody (3D5) [H00000993-M01] - Analysis of protein-protein interactions between CCNB1 and CDC25A. Mahlavu cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-CDC25A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: CDC25A Antibody (3D5) [H00000993-M01] - Detection limit for recombinant GST tagged CDC25A is approximately 0.03ng/ml as a capture antibody.
Applications for CDC25A Antibody (3D5)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: CDC25A
Long Name
Cell Division Cycle 25A
Alternate Names
CDC25A2, CDC25A2-CAG isoform, cell division cycle 25 homolog A (S. cerevisiae), cell division cycle 25 homolog A (S. pombe), cell division cycle 25A, Dual specificity phosphatase Cdc25A, EC 3.1.3.48, M-phase inducer phosphatase 1
Entrez Gene IDs
993 (Human)
Gene Symbol
CDC25A
OMIM
116947 (Human)
UniProt
Additional CDC25A Products
Product Documents for CDC25A Antibody (3D5)
Product Specific Notices for CDC25A Antibody (3D5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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