CDC25B Antibody (4D5S10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16877

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16877-100ul
NBP3-16877-20ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4D5S10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CDC25B Antibodies »

Product Summary for CDC25B Antibody (4D5S10)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CDC25B (P30305). SSESSESSDAGLCMDSPSPMDPHMAEQTFEQAIQAASRIIRNEQFAIRRFQSMPVRLLGHSPVLRNITNSQAPDGRRKSEAGSGAASSSGEDKENDGFVFK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDC25B Antibody (4D5S10)

Western Blot: CDC25B Antibody (4D5S10) [NBP3-16877]

Western Blot: CDC25B Antibody (4D5S10) [NBP3-16877]

Western Blot: CDC25B Antibody (4D5S10) [NBP3-16877] - Western blot analysis of extracts of various cell lines, using CDC25B Rabbit mAb (NBP3-16877) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: CDC25B Antibody (4D5S10) [NBP3-16877]

Immunohistochemistry-Paraffin: CDC25B Antibody (4D5S10) [NBP3-16877]

Immunohistochemistry-Paraffin: CDC25B Antibody (4D5S10) [NBP3-16877] - Immunohistochemistry of paraffin-embedded human appendix using CDC25B Rabbit mAb (NBP3-16877) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for CDC25B Antibody (4D5S10)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CDC25B

CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.

Long Name

Cell Division Cycle 25B

Alternate Names

CDC25HU2, cell division cycle 25 homolog B (S. cerevisiae), cell division cycle 25 homolog B (S. pombe), cell division cycle 25B, Dual specificity phosphatase Cdc25B, EC 3.1.3.48, M-phase inducer phosphatase 2

Gene Symbol

CDC25B

Additional CDC25B Products

Product Documents for CDC25B Antibody (4D5S10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CDC25B Antibody (4D5S10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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