CDC25B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32626

Novus Biologicals, part of Bio-Techne
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NBP2-32626
NBP2-32626-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CDC25B Antibodies »

Product Summary for CDC25B Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PVLRNITNSQAPDGRRKSEAGSGAASSSGEDKENDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDC25B Antibody

Western Blot: CDC25B Antibody [NBP2-32626]

Western Blot: CDC25B Antibody [NBP2-32626]

Western Blot: CDC25B Antibody [NBP2-32626] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: CDC25B Antibody [NBP2-32626]

Immunohistochemistry-Paraffin: CDC25B Antibody [NBP2-32626]

Immunohistochemistry-Paraffin: CDC25B Antibody [NBP2-32626] - Staining in human lymph node and pancreas tissues using anti-CDC25B antibody. Corresponding CDC25B RNA-seq data are presented for the same tissues.
Immunohistochemistry: CDC25B Antibody [NBP2-32626]

Immunohistochemistry: CDC25B Antibody [NBP2-32626]

Immunohistochemistry: CDC25B Antibody [NBP2-32626] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
View 2 more images

Applications for CDC25B Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDC25B

CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.

Long Name

Cell Division Cycle 25B

Alternate Names

CDC25HU2, cell division cycle 25 homolog B (S. cerevisiae), cell division cycle 25 homolog B (S. pombe), cell division cycle 25B, Dual specificity phosphatase Cdc25B, EC 3.1.3.48, M-phase inducer phosphatase 2

Gene Symbol

CDC25B

Additional CDC25B Products

Product Documents for CDC25B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CDC25B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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