Cdc27 Antibody (7U5A3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16170

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16170-100ul
NBP3-16170-20ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7U5A3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cdc27 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 702-824 of human CDC27 (P30260). NPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cdc27 Antibody (7U5A3)

Western Blot: Cdc27 Antibody (7U5A3) [NBP3-16170]

Western Blot: Cdc27 Antibody (7U5A3) [NBP3-16170]

Western Blot: Cdc27 Antibody (7U5A3) [NBP3-16170] - Western blot analysis of extracts of various cell lines, using Cdc27 Rabbit mAb (NBP3-16170) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for Cdc27 Antibody (7U5A3)

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cdc27

Cdc27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. It is a component of the Anaphase Promoting Complex (APC), which is composed of eight protein subunits and is highly conserved in eucaryotic cells. The APC catalyzes the formation of the cyclin B ubiquitin conjugate that is responsible for the ubiquitin mediated proteolysis of B type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.

Alternate Names

ANAPC3CDC27 homolog, anaphase promoting complex subunit 3, Anaphase-promoting complex subunit 3, anaphase-promoting complex, protein 3, APC 3, APC3cell division cycle 27, CDC27Hs, cell division cycle 27 homolog (S. cerevisiae), cell division cycle protein 27 homolog, D0S1430E, D17S978E, HNUC, H-NUC, NUC2, nuc2 homolog

Gene Symbol

CDC27

Additional Cdc27 Products

Product Documents for Cdc27 Antibody (7U5A3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cdc27 Antibody (7U5A3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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