CDC2L2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-496
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 730 and the C-terminus (residue 780) of human Cell Division Cycle 2-like 2 using the numbering given in SwissProt entry Q9UQ88 (GeneID 985).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CDC2L2 Antibody
Western Blot: CDC2L2 Antibody [NB100-496]
Western Blot: CDC2L2 Antibody [NB100-496] - Whole cell lysate (15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDK11 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Immunohistochemistry-Paraffin: CDC2L2 Antibody [NB100-496]
Immunohistochemistry-Paraffin: CDC2L2 Antibody [NB100-496] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-CDK11 (NB100-496). Detection: DABImmunoprecipitation: CDC2L2 Antibody [NB100-496]
Immunoprecipitation: CDC2L2 Antibody [NB100-496] - Detection of human CDK11 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDK11 antibody NB100-496 (lot NB100-496-3) used for IP at 3 ug per reaction. CDK11 was also immunoprecipitated by a previous lot of this antibody (lot NB100-496-2). For blotting immunoprecipitated CDK11, NB100-496 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Applications for CDC2L2 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:500-1:2000
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CDC2L2
Long Name
Cyclin-dependent kinase 11A
Alternate Names
CDC2L3, CDK11A, PITSLREB
Entrez Gene IDs
728642 (Human)
Gene Symbol
CDK11A
UniProt
Additional CDC2L2 Products
Product Documents for CDC2L2 Antibody
Product Specific Notices for CDC2L2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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