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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 730 and the C-terminus (residue 780) of human Cell Division Cycle 2-like 2 using the numbering given in SwissProt entry Q9UQ88 (GeneID 985).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDC2L2 Antibody

Western Blot: CDC2L2 Antibody [NB100-496]

Western Blot: CDC2L2 Antibody [NB100-496]

Western Blot: CDC2L2 Antibody [NB100-496] - Whole cell lysate (15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDK11 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: CDC2L2 Antibody [NB100-496]

Immunohistochemistry-Paraffin: CDC2L2 Antibody [NB100-496]

Immunohistochemistry-Paraffin: CDC2L2 Antibody [NB100-496] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-CDK11 (NB100-496). Detection: DAB
Immunoprecipitation: CDC2L2 Antibody [NB100-496]

Immunoprecipitation: CDC2L2 Antibody [NB100-496]

Immunoprecipitation: CDC2L2 Antibody [NB100-496] - Detection of human CDK11 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDK11 antibody NB100-496 (lot NB100-496-3) used for IP at 3 ug per reaction. CDK11 was also immunoprecipitated by a previous lot of this antibody (lot NB100-496-2). For blotting immunoprecipitated CDK11, NB100-496 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for CDC2L2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CDC2L2

CDK11 is a protein kinase belonging to a large molecular weight protein complex that contains RNA polymerase II. It is associated with the cyclin L regulatory partner and plays a role in influencing transcription and RNA-processing events.

Long Name

Cyclin-dependent kinase 11A

Alternate Names

CDC2L3, CDK11A, PITSLREB

Entrez Gene IDs

728642 (Human)

Gene Symbol

CDK11A

UniProt

Additional CDC2L2 Products

Product Documents for CDC2L2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CDC2L2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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