CDC42 Antibody (JJ086-04)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67895
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JJ086-04
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within Human CDC42 aa 30-191 / 191. (SwissProt: P60953 Human; SwissProt: P60766 Mouse; SwissProt: Q8CFN2 Rat)
Localization
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CDC42 Antibody (JJ086-04)
Western Blot: CDC42 Antibody (JJ086-04) [NBP2-67895]
Western Blot: CDC42 Antibody (JJ086-04) [NBP2-67895] - Western blot analysis of CDC42 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: Jurkat cell lysateImmunohistochemistry-Paraffin: CDC42 Antibody (JJ086-04) [NBP2-67895]
Immunohistochemistry-Paraffin: CDC42 Antibody (JJ086-04) [NBP2-67895] - Analysis of paraffin-embedded mouse pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin.Flow Cytometry: CDC42 Antibody (JJ086-04) [NBP2-67895]
Flow Cytometry: CDC42 Antibody (JJ086-04) [NBP2-67895] - Analysis of Hela cells with CDC42 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Applications for CDC42 Antibody (JJ086-04)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CDC42
Long Name
Cell Division Cycle 42
Alternate Names
G25K
Gene Symbol
CDC42
Additional CDC42 Products
Product Documents for CDC42 Antibody (JJ086-04)
Product Specific Notices for CDC42 Antibody (JJ086-04)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...