CDC42 Antibody (M152)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23212

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M152

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CDC42 Antibodies »

Product Summary for CDC42 Antibody (M152)

Immunogen

Clone (M152) was generated from a rat recombinant Cdc42 protein. This sequence is highly conserved in human and mouse Cdc42. [UniProt# Q8CFN2]

Specificity

The antibody detects a 21 kDa protein in human Jurkat cells and mouse brain. It does not recognize a human RhoA GST fusion protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for CDC42 Antibody (M152)

CDC42 Antibody (M152)

Immunocytochemistry/Immunofluorescence: CDC42 Antibody (M152) [NBP3-23212] -

Immunocytochemistry/Immunofluorescence: CDC42 Antibody (M152) [NBP3-23212] - Immunocytochemical labeling in rat PC12 cells grown for 4 days on poly-D-lysine-coated plates in the presence (200 ng/ml) or absence (Control) of Nerve Growth Factor (NGF). Anti-Cdc42 (NBP3-23212) was used at 1:50 dilution followed by labeling with donkey anti-mouse:Cy2.
CDC42 Antibody (M152)

Western Blot: CDC42 Antibody (M152) [NBP3-23212] -

Western Blot: CDC42 Antibody (M152) [NBP3-23212] - Western blot analysis of human jurkat cells (lanes 1 & 2) and mouse brain (lanes 3 & 4). The blots were probed with anti-Cdc42 antibody at 1:125 (lanes 1 & 3) or 1:500 (lanes 2 & 4).

Applications for CDC42 Antibody (M152)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: CDC42

CDC42 is a small GTPase of the Rho subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc42, and is able to complement the yeast cdc42 1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott Aldrich syndrome protein (NWASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in at least two transcript variants.

Long Name

Cell Division Cycle 42

Alternate Names

G25K

Gene Symbol

CDC42

Additional CDC42 Products

Product Documents for CDC42 Antibody (M152)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CDC42 Antibody (M152)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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