CDC42EP1 Antibody (4P7S6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15881

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15881-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4P7S6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CDC42EP1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 292-391 of human CDC42EP1 (Q00587). AGLGPVAEVKSSPVGGGPRGPAGPALGRHWGAGWDGGHHYPEMDARQERVEVLPQARASWESLDEEWRAPQAGSRTPVPSTVQANTFEFADAEEDDEVKV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDC42EP1 Antibody (4P7S6)

Western Blot: CDC42EP1 Antibody (4P7S6) [NBP3-15881]

Western Blot: CDC42EP1 Antibody (4P7S6) [NBP3-15881]

Western Blot: CDC42EP1 Antibody (4P7S6) [NBP3-15881] - Western blot analysis of extracts of various cell lines, using CDC42EP1 Rabbit mAb (NBP3-15881) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: CDC42EP1 Antibody (4P7S6) [NBP3-15881]

Immunohistochemistry-Paraffin: CDC42EP1 Antibody (4P7S6) [NBP3-15881]

Immunohistochemistry-Paraffin: CDC42EP1 Antibody (4P7S6) [NBP3-15881] - Immunohistochemistry of paraffin-embedded mouse heart using CDC42EP1 Rabbit mAb (NBP3-15881) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: CDC42EP1 Antibody (4P7S6) [NBP3-15881]

Immunohistochemistry-Paraffin: CDC42EP1 Antibody (4P7S6) [NBP3-15881]

Immunohistochemistry-Paraffin: CDC42EP1 Antibody (4P7S6) [NBP3-15881] - Immunohistochemistry of paraffin-embedded rat heart using CDC42EP1 Rabbit mAb (NBP3-15881) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for CDC42EP1 Antibody (4P7S6)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CDC42EP1

CDC42EP1, also known as CDC42 Effector Protein 1, consists of a 40.2 kDa and 39.7 kDa isoform, and is involved in regulatory processes within the cell as a member of the Rho GTPase family. Most commonly found in bone marrow, the CDC42EP1 proteins been shown to help facilitate actin cytoskeleton restructuring and cell shape regulation. The protein is being studied for its involvement in osteonecrosis. The protein has been linked to the RhoA and Rho GTPases pathways where it interacts with MAPKAP1, RPTOR, RHOQ, CDC42, RBPMS, KRTAP4-12, PLSCR1.

Alternate Names

Binder of Rho GTPases 5, Borg5, BORG5cdc42 effector protein 1, CDC42 effector protein (Rho GTPase binding) 1, CEP155 kDa bone marrow stromal/endothelial cell protein, MSE55MGC15316, serum constituent protein, Serum protein MSE55

Gene Symbol

CDC42EP1

Additional CDC42EP1 Products

Product Documents for CDC42EP1 Antibody (4P7S6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CDC42EP1 Antibody (4P7S6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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