CDC42EP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88379

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-88379
NBP1-88379-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CDC42EP1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SGFCTISRLPRSEKPHDRDRDGSFPSEPGLRRSDSLLSFRLDLDLGPSLLSELLGVMSLPEAPAAETPAPAANPPAPTANPTGPAANPPATTANPPAPAANPSAPAATPTGPAANPPAPAASSTPHGHCPNGV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDC42EP1 Antibody

Western Blot: CDC42EP1 Antibody [NBP1-88379]

Western Blot: CDC42EP1 Antibody [NBP1-88379]

Western Blot: CDC42EP1 Antibody [NBP1-88379] - HeLa whole cell lysate. Lanes 1 and 4: HeLa cells transfected with control non-targeting siRNA. Lanes 2 and 3: HeLa cells transfected with two different siRNA-targeting Cdc42EP1. Blot probed with cdc42ep1 antibody and reprobed with GAPDH as loading control. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: CDC42EP1 Antibody [NBP1-88379]

Immunocytochemistry/ Immunofluorescence: CDC42EP1 Antibody [NBP1-88379]

Immunocytochemistry/Immunofluorescence: CDC42EP1 Antibody [NBP1-88379] - Confocal Imunofluorescent images of HeLa cells fixed with PFA and stained with Cdc42EP1 antibody at 1:200 (magenta), and phalloidin-Alexa 488 (green) to visualize F-actin. Antibody stains sub nuclear filaments with some co-loclization to actin filaments. Image from verified customer review.
Immunohistochemistry-Paraffin: CDC42EP1 Antibody [NBP1-88379]

Immunohistochemistry-Paraffin: CDC42EP1 Antibody [NBP1-88379]

Immunohistochemistry-Paraffin: CDC42EP1 Antibody [NBP1-88379] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.
View 2 more images

Applications for CDC42EP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Immunoprecipitation

Validated from verified customer review

Western Blot

Validated from verified customer review
Application Notes
For IHC-Paraffin, HIER pH 6 antigen retrieval is recommended. ICC/IF fixation Permeabilization, Use PFA/Triton X-100.

Reviewed Applications

Read 3 reviews rated 5 using NBP1-88379 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDC42EP1

CDC42EP1, also known as CDC42 Effector Protein 1, consists of a 40.2 kDa and 39.7 kDa isoform, and is involved in regulatory processes within the cell as a member of the Rho GTPase family. Most commonly found in bone marrow, the CDC42EP1 proteins been shown to help facilitate actin cytoskeleton restructuring and cell shape regulation. The protein is being studied for its involvement in osteonecrosis. The protein has been linked to the RhoA and Rho GTPases pathways where it interacts with MAPKAP1, RPTOR, RHOQ, CDC42, RBPMS, KRTAP4-12, PLSCR1.

Alternate Names

Binder of Rho GTPases 5, Borg5, BORG5cdc42 effector protein 1, CDC42 effector protein (Rho GTPase binding) 1, CEP155 kDa bone marrow stromal/endothelial cell protein, MSE55MGC15316, serum constituent protein, Serum protein MSE55

Gene Symbol

CDC42EP1

Additional CDC42EP1 Products

Product Documents for CDC42EP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CDC42EP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.