CDCA3 Antibody (1J5L2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16514

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16514-100ul
NBP3-16514-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1J5L2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CDCA3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CDCA3 (Q99618). MGSAKSVPVTPARPPPHNKHLARVADPRSPSAGILRTPIQVESSPQPGLPAGEQLEGLKHAQDSDPRSPTLGIARTPMKTSSGDPPSPLVKQLSEVFETE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDCA3 Antibody (1J5L2)

Western Blot: CDCA3 Antibody (1J5L2) [NBP3-16514]

Western Blot: CDCA3 Antibody (1J5L2) [NBP3-16514]

Western Blot: CDCA3 Antibody (1J5L2) [NBP3-16514] - Western blot analysis of extracts of K-562 cells, using CDCA3 Rabbit mAb (NBP3-16514) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: CDCA3 Antibody (1J5L2) [NBP3-16514]

Immunohistochemistry-Paraffin: CDCA3 Antibody (1J5L2) [NBP3-16514]

Immunohistochemistry-Paraffin: CDCA3 Antibody (1J5L2) [NBP3-16514] - Immunohistochemistry of paraffin-embedded human liver cancer using CDCA3 Rabbit mAb (NBP3-16514) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for CDCA3 Antibody (1J5L2)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CDCA3

CDCA3/TOME1 is an F-box protein and is part of the SCF complex, an ubiquitin ligase complex that promotes degradation of the mitosis-inhibitory kinase, wee1. CDCA3/TOME1 expression is cell cycle regulated by cis-promoter elements and activated at the G2/M phase. CDCA3/TOME1 levels are also regulated at the protein level via degradation by the APC (anaphase-promoting complex) during G1.

Alternate Names

C8, cell division cycle associated 3, FBXO52, Gene-rich cluster protein C8, GRCC8cell division cycle-associated protein 3, MGC2577, TOME1, TOME-1gene rich cluster, C8, trigger of mitotic entry 1, Trigger of mitotic entry protein 1

Gene Symbol

CDCA3

Additional CDCA3 Products

Product Documents for CDCA3 Antibody (1J5L2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CDCA3 Antibody (1J5L2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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