CDCA3 Antibody (4A10)

Novus Biologicals, part of Bio-Techne | Catalog # H00083461-M10

Novus Biologicals, part of Bio-Techne
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H00083461-M10
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4A10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CDCA3 Antibodies »

Product Specifications

Immunogen

CDCA3 (AAH02551, 1 a.a. ~ 268 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGSAKSVPVTPARPPPHNKHLARVADPRSPSAGILRTPIQVESSPQPGLPAGEQLEGLKHAQDSDPRSPTLGIARTPMKTSSGDPPSPLVKQLSEVFETEDSKSNLPPEPVLPPEAPLSSELDLPLGTQLSVEEQMPPWNQTEFPSKQVFSKEEARQPTETPVASQSSDKPSRDPETPRSSGSMRNRWKPNSSKVLGRSPLTILQDDNSPGTLTLRQGKRPSPLSENVSELKEGAILGTGRLLKTGGRAWEQGQDHDKENQHFPLVES

Reactivity Notes

Human. Other species not tested.

Specificity

cell division cycle associated 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for CDCA3 Antibody (4A10)

ELISA: CDCA3 Antibody (4A10) [H00083461-M10]

ELISA: CDCA3 Antibody (4A10) [H00083461-M10]

ELISA: CDCA3 Antibody (4A10) [H00083461-M10] - Detection limit for recombinant GST tagged CDCA3 is approximately 3ng/ml as a capture antibody.

Applications for CDCA3 Antibody (4A10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CDCA3

CDCA3/TOME1 is an F-box protein and is part of the SCF complex, an ubiquitin ligase complex that promotes degradation of the mitosis-inhibitory kinase, wee1. CDCA3/TOME1 expression is cell cycle regulated by cis-promoter elements and activated at the G2/M phase. CDCA3/TOME1 levels are also regulated at the protein level via degradation by the APC (anaphase-promoting complex) during G1.

Alternate Names

C8, cell division cycle associated 3, FBXO52, Gene-rich cluster protein C8, GRCC8cell division cycle-associated protein 3, MGC2577, TOME1, TOME-1gene rich cluster, C8, trigger of mitotic entry 1, Trigger of mitotic entry protein 1

Entrez Gene IDs

83461 (Human)

Gene Symbol

CDCA3

OMIM

607749 (Human)

UniProt

AAH02551 (Human)

Additional CDCA3 Products

Product Documents for CDCA3 Antibody (4A10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CDCA3 Antibody (4A10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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