CDCA3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83332

Novus Biologicals, part of Bio-Techne
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NBP1-83332
NBP1-83332-25ul
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CDCA3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:KEEARQPTETPVASQSSDKPSRDPETPRSSGSMRNRWKPNSSKVLGRSPLTILQDDNSPGTLTLRQGKRPSPLSENVSELKEGAILGTGRLLKTGGRAWEQ

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDCA3 Antibody

Western Blot: CDCA3 Antibody [NBP1-83332]

Western Blot: CDCA3 Antibody [NBP1-83332]

Western Blot: CDCA3 Antibody [NBP1-83332] - Analysis in human cell line A-431.
Western Blot: CDCA3 Antibody [NBP1-83332]

Western Blot: CDCA3 Antibody [NBP1-83332]

Western Blot: CDCA3 Antibody [NBP1-83332] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for CDCA3 Antibody

Application
Recommended Usage

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDCA3

CDCA3/TOME1 is an F-box protein and is part of the SCF complex, an ubiquitin ligase complex that promotes degradation of the mitosis-inhibitory kinase, wee1. CDCA3/TOME1 expression is cell cycle regulated by cis-promoter elements and activated at the G2/M phase. CDCA3/TOME1 levels are also regulated at the protein level via degradation by the APC (anaphase-promoting complex) during G1.

Alternate Names

C8, cell division cycle associated 3, FBXO52, Gene-rich cluster protein C8, GRCC8cell division cycle-associated protein 3, MGC2577, TOME1, TOME-1gene rich cluster, C8, trigger of mitotic entry 1, Trigger of mitotic entry protein 1

Gene Symbol

CDCA3

Additional CDCA3 Products

Product Documents for CDCA3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CDCA3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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