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CDCA3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98988

Novus Biologicals, part of Bio-Techne
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NBP2-98988-100ul
NBP2-98988-50ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human CDCA3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CDCA3 Antibody

Immunocytochemistry/ Immunofluorescence: CDCA3 Antibody [NBP2-98988]

Immunocytochemistry/ Immunofluorescence: CDCA3 Antibody [NBP2-98988]

Immunocytochemistry/Immunofluorescence: CDCA3 Antibody [NBP2-98988] - Immunofluorescence staining of CDCA3 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CDCA3 polyclonal antibody (dilution ratio: 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to cytoplasm.
Immunohistochemistry-Paraffin: CDCA3 Antibody [NBP2-98988]

Immunohistochemistry-Paraffin: CDCA3 Antibody [NBP2-98988]

Immunohistochemistry-Paraffin: CDCA3 Antibody [NBP2-98988] - Immunochemical staining of human CDCA3 in human colon carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: CDCA3 Antibody [NBP2-98988]

Immunohistochemistry-Paraffin: CDCA3 Antibody [NBP2-98988]

Immunohistochemistry-Paraffin: CDCA3 Antibody [NBP2-98988] - Immunochemical staining of human CDCA3 in human brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Applications for CDCA3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDCA3

CDCA3/TOME1 is an F-box protein and is part of the SCF complex, an ubiquitin ligase complex that promotes degradation of the mitosis-inhibitory kinase, wee1. CDCA3/TOME1 expression is cell cycle regulated by cis-promoter elements and activated at the G2/M phase. CDCA3/TOME1 levels are also regulated at the protein level via degradation by the APC (anaphase-promoting complex) during G1.

Alternate Names

C8, cell division cycle associated 3, FBXO52, Gene-rich cluster protein C8, GRCC8cell division cycle-associated protein 3, MGC2577, TOME1, TOME-1gene rich cluster, C8, trigger of mitotic entry 1, Trigger of mitotic entry protein 1

Gene Symbol

CDCA3

Additional CDCA3 Products

Product Documents for CDCA3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CDCA3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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