CDK4 Antibody (5-C3-G3-R)

Western Blot: CDK4 Antibody (5-C3-G3-R) [NBP3-32175]

Western Blot: CDK4 Antibody (5-C3-G3-R) [NBP3-32175] - Western blot analysis of CDK4 on different lysates with Mouse anti-CDK4 antibody (NBP3-32175) at 1/1,000 dilution.

Lane 1: A549 cell lysate
Lane 2: 293T cell lysate
Lane 3: HeLa cell lysate
Lane 4: MCF7 cell lysate
Lane 5: K-562 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 34 kDa
Observed band size: 34 kDa

Exposure time: 17 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32175) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.