CDK5 Activator 1 Antibody (4G11)

Novus Biologicals, part of Bio-Techne | Catalog # H00008851-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4G11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CDK5 Activator 1 Antibodies »

Product Specifications

Immunogen

CDK5R1 (AAH20580, 208 a.a. ~ 307 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CRDVISSEVGSDHELQAVLLTCLYLSYSYMGNEISYPLKPFLVESCKEAFWDRCLSVINLMSSKMLQINADPHYFTQVFSDLKNESGQEDKKRLLLGLDR

Specificity

CDK5R1 - cyclin-dependent kinase 5, regulatory subunit 1 (p35)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CDK5 Activator 1 Antibody (4G11)

Western Blot: CDK5 Activator 1 Antibody (4G11) [H00008851-M01]

Western Blot: CDK5 Activator 1 Antibody (4G11) [H00008851-M01]

Western Blot: CDK5 Activator 1 Antibody (4G11) [H00008851-M01] - Analysis of CDK5R1 expression in transfected 293T cell line by CDK5R1 monoclonal antibody (M01), clone 4G11.Lane 1: CDK5R1 transfected lysate(34.1 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: CDK5 Activator 1 Antibody (4G11) [H00008851-M01]

Immunocytochemistry/ Immunofluorescence: CDK5 Activator 1 Antibody (4G11) [H00008851-M01]

Immunocytochemistry/Immunofluorescence: CDK5 Activator 1 Antibody (4G11) [H00008851-M01] - Analysis of monoclonal antibody to CDK5R1 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: CDK5 Activator 1 Antibody (4G11) [H00008851-M01] - Detection limit for recombinant GST tagged CDK5R1 is approximately 0.3ng/ml as a capture antibody.

Applications for CDK5 Activator 1 Antibody (4G11)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CDK5 Activator 1

The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease.

Long Name

Cyclin-dependent Kinase 5, Regulatory Subunit 1 (p35)

Alternate Names

CDK5P35, CDK5R1, NCK5A, Neuronal CDK5 Activator, p35nck5a, TPKII Regulatory Subunit

Entrez Gene IDs

8851 (Human)

Gene Symbol

CDK5R1

OMIM

603460 (Human)

UniProt

AAH20580 (Human)

Additional CDK5 Activator 1 Products

Product Documents for CDK5 Activator 1 Antibody (4G11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CDK5 Activator 1 Antibody (4G11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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