Cdk5 Antibody (1H3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47638

Novus Biologicals, part of Bio-Techne
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NBP1-47638
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1H3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CDK5 Antibodies »

Product Summary for Cdk5 Antibody (1H3)

Immunogen

Purified rat Cdk5. Accession # Q03114

Reactivity Notes

Tested in Human, Mouse and Rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

The antibody is specific for the ~ 28kDa Cdk5 protein in Western blots of rat striatal lysate. The antibody shows no cross reactivity with the Cdk5 cofactor p35 or its degradation product p25. This monoclonal also works well for immunocytochemistry using primary cultured rat neurons and for immunohistochemistry on mouse brain tissue.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cdk5 Antibody (1H3)

Western Blot: Cdk5 Antibody (1H3) [NBP1-47638]

Western Blot: Cdk5 Antibody (1H3) [NBP1-47638]

Western Blot: Cdk5 Antibody [NBP1-47638] - Rat Caudate Lysate showing specific immunolabeling of the ~28k Cdk5 protein.

Applications for Cdk5 Antibody (1H3)

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CDK5

Cyclin-dependent protein kinase 5 (Cdk5) is a unique member of the family of cyclin-dependent kinases that is not directly involved in the progression of proliferating cells through the cell cycle (1). Distributed in most tissues and various cell lines, Cdk5 is active primarily in neuronal tissues, because of its association with neuron-specific activators such as p35 (a neural-specific regulatory subunit of Cdk5) (2). In addition to its role in muscle and neuronal differentiation, Cdk5 also contributes to the assembly, organization and stability of the axonal cytoskeleton in neurons, promotes neurite outgrowth and axon guidance in developing neurons, participates in the regulation of synaptic transmission and provides an additional pathway to cellular apoptosis (3-5). Cdk5 phosphorylates MAP1b, Tau, neurofilament proteins, caldesmon and proteins of the synaptic vesicles. Deregulation of Cdk5 has profound cytotoxic effects and is implicated in the development of neurodegenerative diseases such as Alzheimer's disease and amyotrophic lateral sclerosis (6).

Long Name

Cyclin-dependent Kinase 5

Alternate Names

CDKN5, LIS7, PSSALRE

Entrez Gene IDs

140908 (Rat)

Gene Symbol

CDK5

UniProt

Q03114 (Rat)

Additional CDK5 Products

Product Documents for Cdk5 Antibody (1H3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cdk5 Antibody (1H3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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