Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens cyclin-dependent kinase 5 (CDK5), transcript variant 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

33.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cdk5 Antibody (OTI2H6)

Western Blot: Cdk5 Antibody (2H6) [NBP1-47692] Analysis of extracts (35ug) from 9 different cell lines by using anti-Cdk5 monoclonal antibody.

Immunocytochemistry/ Immunofluorescence: Cdk5 Antibody (OTI2H6) [NBP1-47692]

Immunocytochemistry/Immunofluorescence: Cdk5 Antibody (2H6) [NBP1-47692] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cdk5.

Flow Cytometry: Cdk5 Antibody (OTI2H6) [NBP1-47692]

Flow Cytometry: Cdk5 Antibody (2H6) [NBP1-47692] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Cdk5 antibody, and then analyzed by flow cytometry.

View 2 more images

Applications for Cdk5 Antibody (OTI2H6)

Application

Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-100

Western Blot

1:1000-2000

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CDK5

Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase

Long Name

Cyclin-dependent Kinase 5

Alternate Names

CDKN5, LIS7, PSSALRE

Entrez Gene IDs

1020 (Human)

Gene Symbol

CDK5

Additional CDK5 Products

Product Documents for Cdk5 Antibody (OTI2H6)

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Cdk5 Antibody (OTI2H6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Cdk5 Antibody (OTI2H6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47692

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

Concentration

View all CDK5 Antibodies »

Product Summary for Cdk5 Antibody (OTI2H6)

Immunogen

Reactivity Notes

Specificity

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for Cdk5 Antibody (OTI2H6)

Immunocytochemistry/ Immunofluorescence: Cdk5 Antibody (OTI2H6) [NBP1-47692]

Flow Cytometry: Cdk5 Antibody (OTI2H6) [NBP1-47692]

Western Blot: Cdk5 Antibody (OTI2H6) [NBP1-47692]

Immunocytochemistry/ Immunofluorescence: Cdk5 Antibody (OTI2H6) [NBP1-47692]

Applications for Cdk5 Antibody (OTI2H6)

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: CDK5

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

Additional CDK5 Products

Product Documents for Cdk5 Antibody (OTI2H6)

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Cdk5 Antibody (OTI2H6)