CDT2 Antibody (JE58-01)

Western Blot: CDT2 Antibody (JE58-01) [NBP3-32176]

Western Blot: CDT2 Antibody (JE58-01) [NBP3-32176] - Western blot analysis of CDT2 on different lysates with Rabbit anti-CDT2 antibody (NBP3-32176) at 1/2,000 dilution.

Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: 293T cell lysate (20 ug/Lane)
Lane 3: K-562 cell lysate (20 ug/Lane)
Lane 4: Jurkat cell lysate (20 ug/Lane)
Lane 5: Mouse spleen tissue lysate (40 ug/Lane)

Predicted band size: 79 kDa
Observed band size: 79 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32176) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: CDT2 Antibody (JE58-01) [NBP3-32176]

Flow Cytometry: CDT2 Antibody (JE58-01) [NBP3-32176] - Flow cytometric analysis of HeLa cells labeling CDT2.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32176, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).