Key Product Details

Species Reactivity

Human

Applications

Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 123a

Format

BSA Free

Concentration

1 mg/ml

View all CDX1 Antibodies »

Product Specifications

Immunogen

Human Cdx1 N-terminal peptide

Specificity

Clone 123a has a degree of cross reactivity with a determinant of different molecular weight when applied by WB and IHC in fibroblasts and smooth muscle cells. The cross reacting product does not appear in epithelial cells (or at least is significantly weaker).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for Cdx1 Antibody (123a) - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): HT55, LS174T, RCM1.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDX1

The transcription factors Cdx1 and Cdx2 are members of the caudal-related homeobox gene family based on their homology to the factor caudal in Drosophila melanogaster. The caudal homeobox factor is required for anterior-posterior regional identity and axial patterning in Drosophila. During mouse development, the expression of Cdx1 and Cdx2 can be divided into two stages that likely reflect distinct roles. During early development, these two factors are expressed in a Hox-like manner in the three germ layers and appear to have overlapping functions in anterior-posterior patterning and posterior axis elongation. The second stage of Cdx1 and Cdx2 expression, from late development into adulthood, is characterized by the loss of expression almost everywhere but the epithelium of small intestine and colon. Putative intestine-specific enhancers upstream of the CDX1 gene have been identified that may orchestrate the switch in expression patterns for that gene. As might be expected from this pattern of expression, a wide variety of in vivo and in vitro studies have suggested that these two factors are important in controlling epithelial cell differentiation, proliferation, and function in the intestine.

Long Name

Caudal-type Homeobox Transcription Factor 1

Alternate Names

caudal type homeo box transcription factor 1, caudal type homeobox 1, caudal type homeobox transcription factor 1, Caudal-type homeobox protein 1, caudal-type homeobox protein CDX1, homeobox protein CDX-1, MGC116915

Gene Symbol

CDX1

Additional CDX1 Products

Product Documents for Cdx1 Antibody (123a) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cdx1 Antibody (123a) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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