Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # CDX2/1690

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CDX2 Antibodies »

Product Summary for CDX2 Antibody (CDX2/1690) - Azide and BSA Free

Immunogen

Recombinant fragment (around aa150-249) of human CDX2 protein (exact sequence is proprietary) (Uniprot: Q99626)

Localization

Nuclear

Marker

GI Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53247).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CDX2 Antibody (CDX2/1690) - Azide and BSA Free

Immunohistochemistry-Paraffin: CDX2 Antibody (CDX2/1690) - Azide and BSA Free [NBP2-54472]

Immunohistochemistry-Paraffin: CDX2 Antibody (CDX2/1690) - Azide and BSA Free [NBP2-54472]

Immunohistochemistry-Paraffin: CDX2 Antibody (CDX2/1690) - Azide and BSA Free [NBP2-54472] - Gastric Adenocarcinoma stained with CDX2 antibody. pH 9 HIER antigen retrieval. Image from verified customer review.
Immunohistochemistry-Paraffin: CDX2 Antibody (CDX2/1690) - Azide and BSA Free [NBP2-54472]

Immunohistochemistry-Paraffin: CDX2 Antibody (CDX2/1690) - Azide and BSA Free [NBP2-54472]

Immunohistochemistry-Paraffin: CDX2 Antibody (CDX2/1690) [NBP2-54472] - Formalin-fixed, paraffin-embedded Human Colon Carcinoma stained with CDX2 Mouse Monoclonal Antibody (CDX2/1690).
Immunohistochemistry-Paraffin: CDX2 Antibody (CDX2/1690) - Azide and BSA Free [NBP2-54472]

Immunohistochemistry-Paraffin: CDX2 Antibody (CDX2/1690) - Azide and BSA Free [NBP2-54472]

Immunohistochemistry-Paraffin: CDX2 Antibody (CDX2/1690) [NBP2-54472] - Formalin-fixed, paraffin-embedded Human Colon Carcinoma stained with CDX2 Mouse Monoclonal Antibody (CDX2/1690).

Applications for CDX2 Antibody (CDX2/1690) - Azide and BSA Free

Application
Recommended Usage

ELISA

2 - 4 ug/ml

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using NBP2-54472 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CDX2

The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear proteins that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. The proteins LMX1 (MIM 600298) and CDX3 are homeodomain proteins that bind an A/T-rich sequence in the insulin promoter and stimulate its transcription (German et al., 1994 [PubMed 7698771]).[supplied by OMIM]

Long Name

Caudal-type Homeobox Transcription Factor 2

Alternate Names

CDX3

Gene Symbol

CDX2

Additional CDX2 Products

Product Documents for CDX2 Antibody (CDX2/1690) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CDX2 Antibody (CDX2/1690) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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