CDX2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24583

Novus Biologicals, part of Bio-Techne
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NBP2-24583
NBP2-24583SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Equine, Opossum, Primate

Predicted:

Monkey (93%), Rhesus Macaque (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all CDX2 Antibodies »

Product Summary for CDX2 Antibody

Immunogen

A portion of amino acids 1-60 of human CDX2 /CDX3 was used as the immunogen for the antibody.

Reactivity Notes

The amino acid sequence used as immunogen for the GATA-4 antibody is 93% homologous in Rhesus Macaque, 87% homologous in Rat, 82% in Mouse, and 81% homologous in Canine.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDX2 Antibody

Western Blot: CDX2 Antibody [NBP2-24583]

Western Blot: CDX2 Antibody [NBP2-24583]

Western Blot: CDX2 Antibody [NBP2-24583] - Total protein from human HeLa, HepG2, CaCo2 and HCT116, mouse 3T3 and Neuro2A and rat PC12 cells lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-CDX2 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.
Immunohistochemistry-Paraffin: CDX2 Antibody [NBP2-24583]

Immunohistochemistry-Paraffin: CDX2 Antibody [NBP2-24583]

Immunohistochemistry-Paraffin: CDX2 Antibody [NBP2-24583] - Formalin-fixed, paraffin-embedded human spleen probed with CDX2 / CDX3 antibody at 5 ug/ml.
Western Blot: CDX2 Antibody [NBP2-24583]

Western Blot: CDX2 Antibody [NBP2-24583]

Western Blot: CDX2 Antibody [NBP2-24583] - Analysis of CDX3/CDX2 in HCT-116 cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Applications for CDX2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1-3 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CDX2

The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear proteins that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. The proteins LMX1 (MIM 600298) and CDX3 are homeodomain proteins that bind an A/T-rich sequence in the insulin promoter and stimulate its transcription (German et al., 1994 [PubMed 7698771]).[supplied by OMIM]

Long Name

Caudal-type Homeobox Transcription Factor 2

Alternate Names

CDX3

Gene Symbol

CDX2

UniProt

Q99626

Additional CDX2 Products

Product Documents for CDX2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CDX2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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