CEACAM5/CD66e Antibody (308/3-3) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54624
![Immunoprecipitation: CEACAM5/CD66e Antibody (308/3-3) - BSA Free [NBP2-54624]](https://resources.bio-techne.com/images/products/CEACAM3-5-Antibody-308-3-3-Immunoprecipitation-NBP2-54624-img0003.jpg "Immunoprecipitation: CEACAM5/CD66e Antibody (308/3-3) - BSA Free [NBP2-54624]")
![Western Blot: CEACAM5/CD66e Antibody (308/3-3)BSA Free [NBP2-54624]](https://resources.bio-techne.com/images/products/CEACAM3-5-Antibody-308-3-3-Western-Blot-NBP2-54624-img0004.jpg "Western Blot: CEACAM5/CD66e Antibody (308/3-3)BSA Free [NBP2-54624]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunoprecipitation, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 308/3-3
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant soluble human CEACAM3-Fc produced in HEK293 cells
Epitope
Reacts to an epitope shared by CEACAM 3 and 5
Specificity
Clone 308/3-3 reacts with human CEACAM3 and CEACAM5, but not human CEACAM1/4/6/7/8, rat CEACAM1, or mouse CEACAM1 & CEACAM2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for CEACAM5/CD66e Antibody (308/3-3) - BSA Free
Western Blot: CEACAM5/CD66e Antibody (308/3-3)BSA Free [NBP2-54624]
Western Blot: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Detection using 10 ug/ml Anti-CEACAM3/5 [308/3-3] followed by HRP-coupled secondary pAb. Visualized by chemiluminescence detection.![Flow Cytometry: CEACAM5/CD66e Antibody (308/3-3) - BSA Free [NBP2-54624]](https://resources.bio-techne.com/images/products/CEACAM3-5-Antibody-308-3-3-Flow-Cytometry-NBP2-54624-img0002.jpg "Flow Cytometry: CEACAM5/CD66e Antibody (308/3-3) - BSA Free [NBP2-54624]")
Flow Cytometry: CEACAM5/CD66e Antibody (308/3-3) - BSA Free [NBP2-54624]
Flow Cytometry: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Binding of 10 ug/ml primary positive control mAb (upper panel), Anti-CEACAM3/5 [308/3-3] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).
Sandwich ELISA: CEACAM3/5 Antibody (308/3-3) [NBP2-54624] - Solid phase was coated with 3 ug/ml Anti-CEA (Dako) binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM3/5 [308/3-3] (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.
Applications for CEACAM5/CD66e Antibody (308/3-3) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10 - 1:1000
Immunoprecipitation
1:10 - 1:500
Sandwich ELISA
1:100 - 1:2000
Western Blot
1:100 - 1:2000
Application Notes
This antibody is Cytof ready.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: CEACAM-5/CD66e
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 5
Alternate Names
CD66e, CEA, CEACAM5
Gene Symbol
CEACAM5
Additional CEACAM-5/CD66e Products
Product Documents for CEACAM5/CD66e Antibody (308/3-3) - BSA Free
Product Specific Notices for CEACAM5/CD66e Antibody (308/3-3) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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