CEACAM5/CD66e Antibody (C66/195) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47973
![Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (C66/195) - Azide and BSA Free [NBP2-47973]](https://resources.bio-techne.com/images/products/CEACAM5-CD66e-Antibody-C66-195-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47973-img0001.jpg "Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (C66/195) - Azide and BSA Free [NBP2-47973]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Monkey
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # C66/195
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Purified human CEACAM5/CD66e protein (Uniprot: P06731)
Localization
Cytoplasmic and luminal surface
Specificity
This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This monoclonal antibody reacts with nonspecific cross-reacting antigen (NCA) and shows a cross-reaction with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbladder, urachus, salivary gland, ovary, and endocervix.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44897).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for CEACAM5/CD66e Antibody (C66/195) - Azide and BSA Free
Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (C66/195) - Azide and BSA Free [NBP2-47973]
Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (C66/195) - Azide and BSA Free [NBP2-47973] - Human Colon Carcinoma stained with CEA Monoclonal Antibody (C66/195)Applications for CEACAM5/CD66e Antibody (C66/195) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
1 - 2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CEACAM-5/CD66e
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 5
Alternate Names
CD66e, CEA, CEACAM5
Gene Symbol
CEACAM5
Additional CEACAM-5/CD66e Products
Product Documents for CEACAM5/CD66e Antibody (C66/195) - Azide and BSA Free
Product Specific Notices for CEACAM5/CD66e Antibody (C66/195) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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