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CEACAM8/CD66b Antibody (80H3)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-64916

Novus Biologicals, part of Bio-Techne
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NB100-64916
NB100-64916-0.025mg

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Flow Cytometry, Functional, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 80H3

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Human leucocytes from individuals with CML

Specificity

NB100-64916 reacts with the CGM6 gene product of the carcinoembryonic gene family. This is a 95-100kD GPI anchored molecule. CEACAM8 is expressed strongly by mature granulocytes and metamyelocytes but weakly by bone marrow myelocytes. CEACAM8 is not expressed by peripheral blood monocytes or lymphocytes.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for CEACAM8/CD66b Antibody (80H3)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:25-1:100

Western Blot

1:100-1:2000
Application Notes
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

<0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CEACAM-8/CD66b

CEACAM8 is expressed in leukocytes of Chronic Myeloid Leukemia patients and bone marrow. It belongs to the immunoglobulin superfamily, CEA family. The antigen is expressed strongly by mature granulocytes and meta myelocytes, but only weakly by bone marrow myelocytes. Peripheral blood monocytes and lymphocytes do not express significant amounts of the antigen.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 8

Alternate Names

CD66b, CD67, CEACAM8, CGM6, NCA-95

Entrez Gene IDs

1088 (Human)

Gene Symbol

CEACAM8

UniProt

Additional CEACAM-8/CD66b Products

Product Documents for CEACAM8/CD66b Antibody (80H3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CEACAM8/CD66b Antibody (80H3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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