Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 47A1

Format

BSA Free

Concentration

1.0 mg/ml

View all CEBP Beta Antibodies »

Product Specifications

Immunogen

Recombinant human CEBP Beta (1-271 aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CEBP Beta Antibody (47A1) - BSA Free

Western Blot: CEBP Beta Antibody (47A1)BSA Free [NBP1-04266]

Western Blot: CEBP Beta Antibody (47A1)BSA Free [NBP1-04266]

Western Blot: CEBP Beta Antibody (47A1) [NBP1-04266] - NIH 3T3 cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CEBP Beta; antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot: CEBP Beta Antibody (47A1)BSA Free [NBP1-04266]

Western Blot: CEBP Beta Antibody (47A1)BSA Free [NBP1-04266]

Western Blot: CEBP Beta Antibody (47A1) [NBP1-04266] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1 hour at room temperature. The membrane was probed with anti-CEBP beta 1:1000 in non-fat milk. This image was submitted via customer review.

Applications for CEBP Beta Antibody (47A1) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:500-1:2000

Reviewed Applications

Read 1 review rated 5 using NBP1-04266 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CEBP Beta

CCAAT/enhancer-binding proteins (C/EBPs) are a family of basic leucine zipper (bZIP) DNA binding proteins that play important roles in normal cellular differentiation and function in a variety of tissues. The C/EBP family is critical for innate immunity and inflammatory response, and consists of five core members: C/EBP alpha, C/EBP beta, C/EBP delta, C/EBP epsilon, and C/EBP gamma. Among these proteins, C/EBP beta is usually highly expressed in liver, intestine, lung and adipose. C/EBP beta has three isoforms, two activator, (LAP* and LAP) and a repressor (LIP). These isoforms are differentially translated from the same mRNA. The expression of these isoforms is developmentally and hormonally regulated.

Alternate Names

C/EBP beta, C/EBP-beta, CCAAT/enhancer binding protein (C/EBP), beta, CCAAT/enhancer-binding protein beta, CRP2, IL6DBP, interleukin 6-dependent DNA-binding protein, LAPTCF-5, Liver activator protein, liver-enriched transcriptional activator protein, MGC32080, NF-IL6, Nuclear factor NF-IL6, nuclear factor of interleukin 6, TCF5NFIL6, Transcription factor 5

Gene Symbol

CEBPB

UniProt

NP_005185 (Human)

Additional CEBP Beta Products

Product Documents for CEBP Beta Antibody (47A1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CEBP Beta Antibody (47A1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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