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CEBP epsilon Antibody (7A4)

Novus Biologicals, part of Bio-Techne | Catalog # H00001053-M01

Novus Biologicals, part of Bio-Techne
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H00001053-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 7A4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

CEBPE (AAH35797.2, 1 a.a. ~ 281 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSHGTYYECEPRGGQQPLEFSGGRAGPGELGDMCEHEASIDLSAYIESGEEQLLSDLFAVKPAPEARGLKGPGTPAFPHYLPPDPRPFAYPPHTFGPDRKALGPGIYSSPGSYDPRAVAVKEEPRGPEGSRAASRGSYNPLQYQVAHCGQTAMHLPPTLAAPGQPLRVLKAPLATAAPPCSPLLKAPSPAGPLHKGKKAVNKDSLEYRLRRERNNIAVRKSRDKAKRRILETQQKVLEYMAENERLRSRVEQLTQELDTLRNLFRQIPEAANLIKGVGGCS

Specificity

CEBPE - CCAAT/enhancer binding protein (C/EBP), epsilon

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CEBP epsilon Antibody (7A4)

Western Blot: CEBP epsilon Antibody (7A4) [H00001053-M01]

Western Blot: CEBP epsilon Antibody (7A4) [H00001053-M01]

Western Blot: CEBP epsilon Antibody (7A4) [H00001053-M01] - Analysis of CEBPE expression in transfected 293T cell line by CEBPE monoclonal antibody (M01), clone 7A4.Lane 1: CEBPE transfected lysate(30.6 KDa).Lane 2: Non-transfected lysate.
Western Blot: CEBP epsilon Antibody (7A4) [H00001053-M01]

Western Blot: CEBP epsilon Antibody (7A4) [H00001053-M01]

Western Blot: CEBP epsilon Antibody (7A4) [H00001053-M01] - Analysis of CEBPE over-expressed 293 cell line, cotransfected with CEBPE Validated Chimera RNAi ( Cat # H00001053-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CEBPE monoclonal antibody (M01), clone 7A4 (Cat # H00001053-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Applications for CEBP epsilon Antibody (7A4)

Application
Recommended Usage

Western Blot

1:500RNAi Validation
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CEBP epsilon

The protein encoded by this gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-delta. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with Specific Granule Deficiency, a rare congenital disorder. Multiple variants of this gene have been described, but the full-length nature of only one has been determined.

Long Name

CCAAT/enhancer-Binding Protein epsilon

Alternate Names

CEBPE

Entrez Gene IDs

1053 (Human)

Gene Symbol

CEBPE

Additional CEBP epsilon Products

Product Documents for CEBP epsilon Antibody (7A4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CEBP epsilon Antibody (7A4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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