CEBP gamma Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-96986

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CEBP gamma Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human CEBP gamma (Accession#: P53567; Pro39-Asn147)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CEBP gamma Antibody

Western Blot: CEBP gamma Antibody [NBP2-96986]

Western Blot: CEBP gamma Antibody [NBP2-96986]

Western Blot: CEBP gamma Antibody [NBP2-96986] - Anti-CEBP gamma rabbit polyclonal antibody at 1:500 dilution. Lane A: Raw264.7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 16 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: CEBP gamma Antibody [NBP2-96986]

Immunoprecipitation: CEBP gamma Antibody [NBP2-96986]

Immunoprecipitation: CEBP gamma Antibody [NBP2-96986] - CEBP gamma was immunoprecipitated using: Lane A: 0.5 mg Raw264.7 Whole Cell Lysate. 2 ul anti-CEBP gamma rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CEBP gamma rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 28 kDa

Applications for CEBP gamma Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CEBP gamma

The transcription factor C/EBP alpha (CCAAT-enhancer binding protein) is a heatstable, sequence-specific DNA-binding protein first purified from rat liver nuclei that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP alpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP alpha uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP alpha appears to function exclusively in terminally differentiated, growth-arrested cells. Additional family members include C/EBP beta, C/EBP gamma,C/EBP delta and C/EBP episilon, all of which exhibit similar DNA-binding specificities and affinities to C/EBP alpha. Furthermore, C/EBP beta and C/EBP delta readily form heterodimers both with each other as well as with C/EBP alpha.

Alternate Names

c/EBP gamma, CCAAT/enhancer binding protein (C/EBP), gamma, CCAAT/enhancer-binding protein gamma, GPE1BP, IG/EBP-1

Gene Symbol

CEBPG

Additional CEBP gamma Products

Product Documents for CEBP gamma Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CEBP gamma Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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