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CENPC Antibody (5E3K5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16305

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16305-100ul
NBP3-16305-20ul

Key Product Details

Species Reactivity

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5E3K5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 380-520 of human CENPC (Q03188). VLDTSYALIGETVNNYRSTKYEMYSKNAEKPSRSKRTIKQKQRRKFMAKPAEEQLDVGQSKDENIHTSHITQDEFQRNSDRNMEEHEEMGNDCVSKKQMPPVGSKKSSTRKDKEESKKKRFSSESKNKLVPEEVTSTVTKS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CENPC Antibody (5E3K5)

Western Blot: CENPC Antibody (5E3K5) [NBP3-16305]

Western Blot: CENPC Antibody (5E3K5) [NBP3-16305]

Western Blot: CENPC Antibody (5E3K5) [NBP3-16305] - Western blot analysis of extracts of various cell lines, using CENPC Rabbit mAb (NBP3-16305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: CENPC Antibody (5E3K5) [NBP3-16305]

Immunocytochemistry/ Immunofluorescence: CENPC Antibody (5E3K5) [NBP3-16305]

Immunocytochemistry/Immunofluorescence: CENPC Antibody (5E3K5) [NBP3-16305] - Immunofluorescence analysis of C6 cells using CENPC Rabbit mAb (NBP3-16305) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for CENPC Antibody (5E3K5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CENPC

Centromere protein C 1 is a centromere autoantigen and a component of the inner kinetochore plate. The protein is required for maintaining proper kinetochore size and a timely transition to anaphase. A putative pseudogene exists on chromosome 12. [provided by RefSeq]

Alternate Names

CENP-C, CENP-C 1, CENPCInterphase centromere complex protein 7, Centromere autoantigen C, centromere autoantigen C1, centromere protein C 1, hcp-4, ICEN7, MIF2

Gene Symbol

CENPC

Additional CENPC Products

Product Documents for CENPC Antibody (5E3K5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CENPC Antibody (5E3K5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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