CENPC Antibody (JG36-15)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75438

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-75438
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JG36-15

Concentration

1 mg/ml

View all CENPC Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human CENPC aa 360-540 / 943. (SwissProt: Q03188 Human; SwissProt: P49452 Mouse; Entrez Gene: 305270 Rat)

Localization

Nucleus, kinetochore, centromere.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

107 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CENPC Antibody (JG36-15)

Western Blot: CENPC Antibody (JG36-15) [NBP2-75438]

Western Blot: CENPC Antibody (JG36-15) [NBP2-75438]

Western Blot: CENPC Antibody (JG36-15) [NBP2-75438] - Western blot analysis of CENPC on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HL-60 cell lysate Lane 2: Daudi cell lysate Lane 3: K562 cell lysate
Immunocytochemistry/ Immunofluorescence: CENPC Antibody (JG36-15) [NBP2-75438]

Immunocytochemistry/ Immunofluorescence: CENPC Antibody (JG36-15) [NBP2-75438]

Immunocytochemistry/Immunofluorescence: CENPC Antibody (JG36-15) [NBP2-75438] - Staining CENPC in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CENPC Antibody (JG36-15) [NBP2-75438]

Immunohistochemistry-Paraffin: CENPC Antibody (JG36-15) [NBP2-75438]

Immunohistochemistry-Paraffin: CENPC Antibody (JG36-15) [NBP2-75438] - Analysis of paraffin-embedded rat heart tissue using anti-CENPC antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
View 2 more images

Applications for CENPC Antibody (JG36-15)

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CENPC

Centromere protein C 1 is a centromere autoantigen and a component of the inner kinetochore plate. The protein is required for maintaining proper kinetochore size and a timely transition to anaphase. A putative pseudogene exists on chromosome 12. [provided by RefSeq]

Alternate Names

CENP-C, CENP-C 1, CENPCInterphase centromere complex protein 7, Centromere autoantigen C, centromere autoantigen C1, centromere protein C 1, hcp-4, ICEN7, MIF2

Gene Symbol

CENPC

Additional CENPC Products

Product Documents for CENPC Antibody (JG36-15)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CENPC Antibody (JG36-15)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.